Regeneración de yemas adventicias en segmentos de hojas y entrenudos de Balfourodendron riedelianum (Engl.) Engl.

Q4 Environmental Science Colombia Forestal Pub Date : 2022-01-01 DOI:10.14483/2256201x.17767

E. Duarte

引用次数: 1

Abstract

La finalidad de este estudio fue evaluar la capacidad de regeneración de yemas adventicias en explantes de segmentos de hojas y entrenudos provenientes de plantas in vitro. Para ello, fueron germinadas semillas de Balfourodendron riedelianum en condiciones in vitro, y luego de un mes los explantes de hojas y entrenudos fueron cultivados en un medio Murashige y Skoog suplementado con combinaciones ANA (0.01 mg.L-1), BAP (0.1 mg.L-1) y TDZ (0.1, 0.01 mg.L-1). Los mejores resultados se obtuvieron cuando los fragmentos de entrenudos fueron cultivados en 0.1 mg.L-1 de BAP y 0.01 mg.L-1 de TDZ, donde el 26.67±5.77 % de los explantes brindaron 12±4.36 % de yemas por explantes. Se concluyó que los explantes de segmentos de hojas y entrenudos de B. riedelianum son aptos para inducir yemas adventicias.

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Balfourodendron riedelianum（英格兰）叶段和节间不定芽的再生。英格兰。

这项研究的目的是评估离体植物叶段和节间外植体中不定芽的再生能力。为此，在体外条件下发芽，一个月后，在Murashige和Skoog培养基中培养叶片和节间外植体，并添加ANA（0.01 mg.l-1）、BAP（0.1 mg.l-1）和TDZ（0.1、0.01 mg.l-1）的组合。当节间碎片在BAP的0.1 mg.l-1和TDZ的0.01 mg.l-1中培养时，获得最佳结果，其中26.67±5.77%的外植体为每个外植体提供12±4.36%的卵黄。因此，Riedelianum B.叶段和节间的外植体适合诱导不定芽。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colombia Forestal Environmental Science-Nature and Landscape Conservation

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Colombia Forestal Journal publishes original manuscripts on themes of the forest field and various aspects of natural resources and the environment, which are discriminated in the categories of research article, review, reflection and technical notes of agreement with the stipulations of COLCIENCIAS for scientific publications. According to the classification of scientific areas of the Organization for Economic Cooperation and Development (OECD), Colombia Forestal Journal belongs to the large area of Agricultural Sciences (4), Agriculture, Forestry and Fisheries (4A) and to the discipline Forestry (4A02). Since its creation in 1978, the journal has been edited and published by the Francisco José de Caldas District University with headquarters in Bogotá. The quality of the content of the journal has been strengthened with the collaboration of national and international external evaluators in a double blind arbitration system and the dissemination has been extended by publishing manuscripts in a language different from Spanish and with inclusion in bibliographical databases such as Scopus. , DOAJ, Scielo, Redalyc, Ebsco, CAB Abstracts of CAB International, Forestry Abstracts, Ulrich''s, Latindex, Publindex. Likewise, it has academic profiles at Mendeley, Academia and Researchgate. The articles that are submitted to Colombia Forestal Journal must be original and unpublished and have not been postulated in other journals.