Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains

Abstract

Objectives: The study was aimed to compare the performance of three different approaches (temocillin disc diffusion test (TDD), immunochromatographic assay, OXA-48 K-SET, and chromogenic assay containing ChromID OXA-48 media) with PCR in a collection of K. pneumoniae producing OXA-48. Materials and methods: A total of 45 carbapenem resistant K. pneumoniae isolates (27 were OXA-48 positive) were included in the study. Polymerase chain reaction (PCR), TDD, OXA-48 K-SET, ChromID OXA-48 media were applied to all isolates. Results: The OXA-48 K-SET was found to be 100% compatible with gold standard PCR for the identification of OXA-48 positive Klebsiella pneumoniae strains. 100% sensitivity and 88.8% specificity were found with the ChromID OXA-48 medium method. Sensitivity and specificity of the TDD assay were found 100% and 77.8%, respectively. Conclusion: The OXA-48 K-Set had excellent performance for detecting OXA-48 carbapenemase and can be used easily and reliably in the routine clinical microbiology laboratory for rapid detection of OXA-48 producing K. pneumoniae isolates, particularly in endemic regions. ChromID media and temocillin disc diffusion testing can be applied in laboratories as part of the identification and screening of carbapenemase-positive Klebsiella pneumoniae.