Enzyme-Linked Immunosorbent Assay of Autoantibodies against the Human β1-Adrenergic Receptor Using Recombinant Antigens

Abstract

E. coli strains are created as producers of recombinant β1-adrenoreceptor epitopes as part of chimeric proteins. The corresponding epitope sequences are located in the C-terminal region of the human heart fatty acid binding protein (hH-FABP) and separated from it by the linker sequence (Gly₄Ser)₃. A solid-phase enzyme-linked immunosorbent assay (ELISA) to detect autoantibodies against the β1-adrenergic receptor in human blood serum based on a recombinant epitope is developed. Blood sera of patients (N = 76) with various diagnoses of cardiopathologies and other diseases are analyzed. A significantly high level of autoantibodies to the β1-adrenergic receptor is detected in some patients with a confirmed diagnosis of cardiovascular diseases, in most cases those with a diagnosis of acute myocardial infarction.