Triple-negative and triple-positive breast cancer cells reciprocally control their growth and migration via the S100A4 pathway

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2022-05-12 DOI:10.1080/19336918.2022.2072554

E. Dukhanina, T. Portseva, A. Dukhanin, S. Georgieva

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引用次数: 1

Abstract

ABSTRACT The study’s aim was to investigate the S100A4-mediated mechanisms of the regulation of tumor cell proliferation and migration in the human triple-positive breast carcinoma cell line MCF-7 (TPBC) and triple-negative breast carcinoma cell line MDA-MB-231 (TNBC). The proliferative activity of TNBC more than doubled during the incubation in the conditioned medium of TPBC. Extracellular S100A4 dose-dependently decreased the proliferative response of TPBC. TPBC negatively impacted the growth of TNBCs during their co-culturing. TPBC significantly decreased the migration activity of the TNBC cells while the S100A4 intracellular level in the TNBC was also decreasing. The decrease in the S100A4 intracellular level occurred due to the protein’s monomeric form while the contribution of the dimeric form into the overall S100A4 concentration in TNBC cells increased 1.5-2-fold. The S100A4 pathway in the intercellular communication between TNBC and TPBCs also included the dexamethasone-sensitive mechanisms of S100A4 intra- and extracellular pools regulation.

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三阴性和三阳性乳腺癌细胞通过S100A4途径相互控制其生长和迁移

摘要本研究旨在探讨S100A4介导的人类三阳性乳腺癌细胞系MCF-7（TPBC）和三阴性乳腺癌细胞株MDA-MB-231（TNBC）中肿瘤细胞增殖和迁移的调控机制。在TPBC的条件培养基中培养期间，TNBC的增殖活性增加了一倍以上。细胞外S100A4剂量依赖性地降低了TPBC的增殖反应。TPBC对TNBC在共培养过程中的生长产生负面影响。TPBC显著降低了TNBC细胞的迁移活性，而TNBC中的S100A4细胞内水平也在降低。S100A4细胞内水平的下降是由于蛋白质的单体形式，而二聚体形式对TNBC细胞中S100A4总浓度的贡献增加了1.5-2倍。TNBC和TPBC之间细胞间通讯的S100A4途径还包括S100A4胞内和胞外池调节的地塞米松敏感机制。

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