Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.)

IF 1.7 4区农林科学 Q2 HORTICULTURE Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI:10.1080/14620316.2018.1449671

G. Jat, A. D. Munshi, T. Behera, H. Choudhary, P. Dash, Amarnath Ravindran, S. Kumari

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引用次数: 16

Abstract

Gynoecious is an important economic trait of cucumber for determinant of earliness and yield, yet genetic mechanism is not well understood for this trait. The experiment was conducted using F₂ mapping population by crossing of PPC-2, a gynoecious and parthenocarpic line with Pusa Uday (monoecious and non-parthenocarpic cultivar). Out of 179 SSR markers screened, 39 markers differentiated the gynoecious and monoecious parents. However, only 17 markers were segregating with F₂ mapping population, those were used for genotyping and linkage map analysis and these markers were placed along with F locus on chromosome 6 covering a total distance of 100.4cM. The SSR markers, SSR13251 and UW020605 were found to be closely linked to gynoecious (F) locus at 1.0 and 4.5 cM, respectively. The segregation of F₂ population of PPC-2 × Pusa Uday and GPC-1 × Punjab Naveen and test crosses for sex type herein suggested that single dominant gene controlled the gynoecious sex expression in cucumber particularly in gynoecious genotypes PPC-2 and GPC-1. Therefore, the monogenic dominant nature of gynoecious sex identified in the present experiment and SSR markers closely linked to the F locus will be useful in marker-assisted backcross breeding for transfering gynoecious trait into horticulturally desirable varieties.

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黄瓜雌核（F）基因座的遗传与分子定位

结实性是决定黄瓜早熟和产量的重要经济性状，但其遗传机制尚不清楚。实验使用F₂ 通过将雌系和单性结实品系PPC-2与Pusa Uday（雌雄同株和非单性结实品种）杂交来定位种群。在筛选到的179个SSR标记中，39个标记区分了雌雄同株和雌雄同株的亲本。然而，只有17个标记与F分离₂ 将SSR标记SSR13251和UW020605分别与6号染色体上的F位点在1.0和4.5cM处紧密连锁。F的分离₂ ×Pusa Uday和GPC-1×Punjab Naveen群体和性型试验杂交表明，单一显性基因控制着黄瓜的雌性性表达，尤其是在雌性基因型PPC-2和GPC-1。因此，本实验确定的雌虫性的单基因显性性质和与F位点紧密相连的SSR标记将有助于标记辅助回交育种，将雌虫性转化为园艺理想品种。

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来源期刊

Journal of Horticultural Science & Biotechnology 农林科学-园艺

CiteScore

3.90

自引率

5.30%

发文量

审稿时长

3 months

期刊介绍： The Journal of Horticultural Science and Biotechnology is an international, peer-reviewed journal, which publishes original research contributions into the production, improvement and utilisation of horticultural crops. It aims to provide scientific knowledge of interest to those engaged in scientific research and the practice of horticulture. The scope of the journal includes studies on fruit and other perennial crops, vegetables and ornamentals grown in temperate or tropical regions and their use in commercial, amenity or urban horticulture. Papers, including reviews, that give new insights into plant and crop growth, yield, quality and response to the environment, are welcome, including those arising from technological innovation and developments in crop genome sequencing and other biotechnological advances.