Pub Date : 2023-11-05DOI: 10.1080/14620316.2023.2272155
Haoxing Xie, Kaifeng Xing, Jian Zhang, Yao Zhao, Jun Rong
ABSTRACTCamellia oleifera Abel. is the dominant woody oil crop under significant development in China. Wild C. oleifera in Lu Mountain is a valuable genetic resource with strong freezing tolerance. With high-throughput sequencing, the genome of wild C. oleifera in Lu Mountain was analysed and 700.3 Gb clean reads were obtained. The genome of wild C. oleifera was estimated as allohexaploid, and its haplotype genome size was about 2.69 Gb-2.79 Gb, with repeat content of 63.01%-73.02% and heterozygosity of 6.30%-7.43%, belonging to a very complex genome. The genomic draft was assembled that contained a total of 6,952,303 scaffolds with N50 length of 1.23 kb, and the overall length was 2.39 Gb with GC content of 40.87%. In the genomic draft, 1,104,618 SSRs were identified; scaffold1096012 and scaffold1779458 were identified as key genes associated with freezing tolerance combined with the transcriptome data of field plus lab experiments. In this study, the genomic background of hexaploid wild C. oleifera in Lu Mountain was revealed. This lays the foundation for obtaining the high-quality chromosome-level reference genome of wild C. oleifera. The identification of SSRs and key genes associated with freezing tolerance may contribute to the efficient exploration and utilisation of this genetic resource.KEYWORDS: Camellia oleiferahigh-throughput sequencinggenome surveygenomic draftsimple sequence repeatfreezing tolerance AcknowledgementsThis research was funded by the National Natural Science Foundation of China (grant no. 32270238).Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementThe data that support the findings in this research are deposited in the short read archive (SRA) databank (https://www.ncbi.nlm.nih.gov/sra) with the accession number PRJNA984951.Author’s contributionXH and RJ designed the experiments. XH conducted the experiments with helps from XK, ZJ, ZY and RJ. XH, XK, ZJ, ZY and RJ participated in data analyses. All authors contributed to writing of the manuscript.Consent for publicationAll the authors have read and consented to submit the manuscript.Ethical approvalThe article does not contain any studies with human participants or animals performed by any of the authors.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2272155Additional informationFundingThis work was supported by the National Natural Science Foundation of China (32270238).
{"title":"Genome survey and identification of key genes associated with freezing tolerance in genomic draft of hexaploid wild <i>Camellia oleifera</i>","authors":"Haoxing Xie, Kaifeng Xing, Jian Zhang, Yao Zhao, Jun Rong","doi":"10.1080/14620316.2023.2272155","DOIUrl":"https://doi.org/10.1080/14620316.2023.2272155","url":null,"abstract":"ABSTRACTCamellia oleifera Abel. is the dominant woody oil crop under significant development in China. Wild C. oleifera in Lu Mountain is a valuable genetic resource with strong freezing tolerance. With high-throughput sequencing, the genome of wild C. oleifera in Lu Mountain was analysed and 700.3 Gb clean reads were obtained. The genome of wild C. oleifera was estimated as allohexaploid, and its haplotype genome size was about 2.69 Gb-2.79 Gb, with repeat content of 63.01%-73.02% and heterozygosity of 6.30%-7.43%, belonging to a very complex genome. The genomic draft was assembled that contained a total of 6,952,303 scaffolds with N50 length of 1.23 kb, and the overall length was 2.39 Gb with GC content of 40.87%. In the genomic draft, 1,104,618 SSRs were identified; scaffold1096012 and scaffold1779458 were identified as key genes associated with freezing tolerance combined with the transcriptome data of field plus lab experiments. In this study, the genomic background of hexaploid wild C. oleifera in Lu Mountain was revealed. This lays the foundation for obtaining the high-quality chromosome-level reference genome of wild C. oleifera. The identification of SSRs and key genes associated with freezing tolerance may contribute to the efficient exploration and utilisation of this genetic resource.KEYWORDS: Camellia oleiferahigh-throughput sequencinggenome surveygenomic draftsimple sequence repeatfreezing tolerance AcknowledgementsThis research was funded by the National Natural Science Foundation of China (grant no. 32270238).Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementThe data that support the findings in this research are deposited in the short read archive (SRA) databank (https://www.ncbi.nlm.nih.gov/sra) with the accession number PRJNA984951.Author’s contributionXH and RJ designed the experiments. XH conducted the experiments with helps from XK, ZJ, ZY and RJ. XH, XK, ZJ, ZY and RJ participated in data analyses. All authors contributed to writing of the manuscript.Consent for publicationAll the authors have read and consented to submit the manuscript.Ethical approvalThe article does not contain any studies with human participants or animals performed by any of the authors.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2272155Additional informationFundingThis work was supported by the National Natural Science Foundation of China (32270238).","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-03DOI: 10.1080/14620316.2023.2268066
Takumi Taguchi, Satoru Motoki
ABSTRACTNewly developed asparagus farming involves planting seedlings in year one and harvesting all emerging spears without allowing the mother ferns to grow. However, changes in asparagus growth over time are unclear. Here, four-year cultivation trials were conducted under different climatic conditions to investigate temporal changes in growth. Plants were monitored weekly. The growing period was divided into three periods: spring and fall, both within optimum growing temperatures, and summer. Spring to the first half of summer revealed two to three peaks of increase in the number of effective stems. The number of effective stems decreased during high temperatures and decreased precipitation periods (summer), and recovered with an increase in precipitation. The number of effective stems displayed peaks during the second half of periods II and III. The increase in stem diameter mostly occurred during summer. Finally, the growing years that occurred with high temperatures and low precipitation in summer showed a significant positive correlation between the yield and maximum stem diameters in spring. The results of this study indicate that plant growth can be used to schedule pest control, irrigation, and fertiliser application for plants with limited resources.KEYWORDS: Asparagus officinalis L.Days after plantingmean air temperaturenumber of effective stemsprecipitationstem diameter AcknowledgementsWe would like to thank Editage (www.editage.com) for English language editing.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementThe data that support the findings of this study are available from the corresponding author, Satoru Motoki, upon reasonable request.Supplementary MaterialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2268066
{"title":"Asparagus stem emergence and growth as influenced by temperature and rainfall in a one-year production system","authors":"Takumi Taguchi, Satoru Motoki","doi":"10.1080/14620316.2023.2268066","DOIUrl":"https://doi.org/10.1080/14620316.2023.2268066","url":null,"abstract":"ABSTRACTNewly developed asparagus farming involves planting seedlings in year one and harvesting all emerging spears without allowing the mother ferns to grow. However, changes in asparagus growth over time are unclear. Here, four-year cultivation trials were conducted under different climatic conditions to investigate temporal changes in growth. Plants were monitored weekly. The growing period was divided into three periods: spring and fall, both within optimum growing temperatures, and summer. Spring to the first half of summer revealed two to three peaks of increase in the number of effective stems. The number of effective stems decreased during high temperatures and decreased precipitation periods (summer), and recovered with an increase in precipitation. The number of effective stems displayed peaks during the second half of periods II and III. The increase in stem diameter mostly occurred during summer. Finally, the growing years that occurred with high temperatures and low precipitation in summer showed a significant positive correlation between the yield and maximum stem diameters in spring. The results of this study indicate that plant growth can be used to schedule pest control, irrigation, and fertiliser application for plants with limited resources.KEYWORDS: Asparagus officinalis L.Days after plantingmean air temperaturenumber of effective stemsprecipitationstem diameter AcknowledgementsWe would like to thank Editage (www.editage.com) for English language editing.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementThe data that support the findings of this study are available from the corresponding author, Satoru Motoki, upon reasonable request.Supplementary MaterialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2268066","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135820200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACTAquaporins (AQPs) are small integral membrane proteins that facilitate the efficient transport of small solutes like water, metalloids, urea, and CO2 across the membrane. Plant aquaporins are known to be involved in vital physiological processes like seed germination, stomatal movement, cell elongation, and reproductive growth. In the present study, 36 AQPs were identified through genome-wide computational analysis performed in the rose genome. Subsequent phylogenetic analysis classified the AQPs into five subfamilies, including 10 Plasma membrane intrinsic proteins (PIPs), 8 Tonoplast intrinsic proteins (TIPs), 9 Nodulin intrinsic proteins (NIPs), 3 Small intrinsic proteins (SIPs), and 6 uncharacterised intrinsic proteins (XIPs). Based on the prediction of silicon solute specificity, one of the NIPs is predicted to be a silicon transporter. Structural analysis revealed the characteristic six trans-membrane domains and two-half alpha helix harbouring two conserved NPA motifs in the majority of the predicted AQPs. The protein tertiary structures predicted with homology-based modelling were used to study the pore lining remains and to predict the solute transport activity of the AQPs. Expression profiling of the rose AQPs showed tissue-specific expression of many members. The data provided here will help in understanding the AQP-based solute transport system in roses and other related species.KEYWORDS: AquaporinsRosaceaerosesolute specificitysilicon AcknowledgementsThe authors are thankful to the “Department of Biotechnology (DBT), Government of India (GoI)”, for the “Ramalingaswami Fellowship Award” to R.D. and H.S. and grants BT/PR32853/AGIII/103/1159/2019 and BT/PR38279/GET/119/351/2020 to H.S.; The Science and Engineering Research Board (SERB), India, Department of Science and Technology (DST), Government of India (GoI), for research grant CRG/2019/006599 awarded to R.D., and H.S., and for research grant SRG/2021/000077 to SN. PR thanks the SERB, Govt. of India for the Project Associate-I fellowship. PK thanks the Director, ICAR-Directorate of Floricultural Research for funding support.Disclosure statementNo potential conflict of interest was reported by the authors.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2272153Data availability statementThe authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.Additional informationFundingThe work was supported by the Department of Biotechnology, Ministry of Science and Technology, India [BT/PR32853/AGIII/103/1159/2019]; Science and Engineering Research Board [SRG/2021/000077].
{"title":"Genome-wide identification and characterisation of <i>Aquaporin</i> s in <i>Rosa chinensis</i>","authors":"Rushil Mandlik, Shivani Sharma, Priyadarshini Rout, Shweta Singh, Gaurav Raturi, Nitika Rana, Humira Sonah, Rupesh Deshmukh, SM Shivaraj, Satyabrata Nanda, Prashant G. Kawar","doi":"10.1080/14620316.2023.2272153","DOIUrl":"https://doi.org/10.1080/14620316.2023.2272153","url":null,"abstract":"ABSTRACTAquaporins (AQPs) are small integral membrane proteins that facilitate the efficient transport of small solutes like water, metalloids, urea, and CO2 across the membrane. Plant aquaporins are known to be involved in vital physiological processes like seed germination, stomatal movement, cell elongation, and reproductive growth. In the present study, 36 AQPs were identified through genome-wide computational analysis performed in the rose genome. Subsequent phylogenetic analysis classified the AQPs into five subfamilies, including 10 Plasma membrane intrinsic proteins (PIPs), 8 Tonoplast intrinsic proteins (TIPs), 9 Nodulin intrinsic proteins (NIPs), 3 Small intrinsic proteins (SIPs), and 6 uncharacterised intrinsic proteins (XIPs). Based on the prediction of silicon solute specificity, one of the NIPs is predicted to be a silicon transporter. Structural analysis revealed the characteristic six trans-membrane domains and two-half alpha helix harbouring two conserved NPA motifs in the majority of the predicted AQPs. The protein tertiary structures predicted with homology-based modelling were used to study the pore lining remains and to predict the solute transport activity of the AQPs. Expression profiling of the rose AQPs showed tissue-specific expression of many members. The data provided here will help in understanding the AQP-based solute transport system in roses and other related species.KEYWORDS: AquaporinsRosaceaerosesolute specificitysilicon AcknowledgementsThe authors are thankful to the “Department of Biotechnology (DBT), Government of India (GoI)”, for the “Ramalingaswami Fellowship Award” to R.D. and H.S. and grants BT/PR32853/AGIII/103/1159/2019 and BT/PR38279/GET/119/351/2020 to H.S.; The Science and Engineering Research Board (SERB), India, Department of Science and Technology (DST), Government of India (GoI), for research grant CRG/2019/006599 awarded to R.D., and H.S., and for research grant SRG/2021/000077 to SN. PR thanks the SERB, Govt. of India for the Project Associate-I fellowship. PK thanks the Director, ICAR-Directorate of Floricultural Research for funding support.Disclosure statementNo potential conflict of interest was reported by the authors.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2272153Data availability statementThe authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.Additional informationFundingThe work was supported by the Department of Biotechnology, Ministry of Science and Technology, India [BT/PR32853/AGIII/103/1159/2019]; Science and Engineering Research Board [SRG/2021/000077].","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134905671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-12DOI: 10.1080/14620316.2023.2263763
Yunyun Xing, Yujie Jiang, Adnan Raza Muhammad, Jianghua Song
ABSTRACTPRRs (pseudo-response regulators) genes play crucial roles in plant growth and development, stress response and other life activities. However, this gene family in cabbage (Brassica oleracea var. capitata L) has yet to be comprehensively studied. To fully understand the status of PRR in cabbage, ten BoPRR genes were extracted from the whole genome of cabbage through bioinformatics analysis, and their gene structure, protein conservative motifs, cis acting elements and collinearity relationships were identified and analysed. Quantitative real-time PCR (qRT-PCR) was used to explore the expression patterns of BoPRR genes in cabbage leaves under various abiotic stresses. Based on phylogenetic analysis, the ten BoPRR genes were divided into three branches, located on four chromosomes. In addition, many photoresponsive elements exist in the promoter region, and specific response elements with low temperature, drought and abscisic acid (ABA). In terms of gene expression patterns, all ten BoPRR genes were found to be expressed in cabbage leaves and showed response to abiotic stress. This study represents the first comprehensive examination of the PRR family in cabbage, providing a theoretical basis for further research on abiotic stress and offering a new gene resource for breeding stress-resistant varieties.KEYWORDS: Abiotic stressBoPRR genescabbagegenome wide analysis AcknowledgementsSJH secured the funding for the project. XYY and SJH conceived and designed the experiments.XYY and JYJ performed the experiments. XYY wrote the paper. SJH and MAR revised themanuscript. All authors read and approved the manuscript.Disclosure statementNo potential conflict of interest was reported by the authors.Data availability statementAll data generated or analysed in this study are included in the articles published here and referenced for supplementary information.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2263763Additional informationFundingThis work was supported by the National Natural Science Foundation of China [No. 32272728, 31872108].
{"title":"Genome-wide identification of pseudo-response regulator (PRR) family members in cabbage ( <i>Brassica oleracea</i> var. <i>capitata</i> L.) and their expression in response to abiotic stress","authors":"Yunyun Xing, Yujie Jiang, Adnan Raza Muhammad, Jianghua Song","doi":"10.1080/14620316.2023.2263763","DOIUrl":"https://doi.org/10.1080/14620316.2023.2263763","url":null,"abstract":"ABSTRACTPRRs (pseudo-response regulators) genes play crucial roles in plant growth and development, stress response and other life activities. However, this gene family in cabbage (Brassica oleracea var. capitata L) has yet to be comprehensively studied. To fully understand the status of PRR in cabbage, ten BoPRR genes were extracted from the whole genome of cabbage through bioinformatics analysis, and their gene structure, protein conservative motifs, cis acting elements and collinearity relationships were identified and analysed. Quantitative real-time PCR (qRT-PCR) was used to explore the expression patterns of BoPRR genes in cabbage leaves under various abiotic stresses. Based on phylogenetic analysis, the ten BoPRR genes were divided into three branches, located on four chromosomes. In addition, many photoresponsive elements exist in the promoter region, and specific response elements with low temperature, drought and abscisic acid (ABA). In terms of gene expression patterns, all ten BoPRR genes were found to be expressed in cabbage leaves and showed response to abiotic stress. This study represents the first comprehensive examination of the PRR family in cabbage, providing a theoretical basis for further research on abiotic stress and offering a new gene resource for breeding stress-resistant varieties.KEYWORDS: Abiotic stressBoPRR genescabbagegenome wide analysis AcknowledgementsSJH secured the funding for the project. XYY and SJH conceived and designed the experiments.XYY and JYJ performed the experiments. XYY wrote the paper. SJH and MAR revised themanuscript. All authors read and approved the manuscript.Disclosure statementNo potential conflict of interest was reported by the authors.Data availability statementAll data generated or analysed in this study are included in the articles published here and referenced for supplementary information.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2263763Additional informationFundingThis work was supported by the National Natural Science Foundation of China [No. 32272728, 31872108].","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135968268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-10DOI: 10.1080/14620316.2023.2247835
Barry J Mulholland, Pardeep S Panesar, Philip H Johnson
Fresh produce handling, particularly in final inspection and pack, is highly dependent on dextrous human labour. As part of a relatively low-profit margin industry, rising wage costs and labour shortages are impacting the viability of this sector and are a direct threat to global food security. Adoption of robotics is required to automate delicate handling tasks; this is a key goal for sustainable and profitable businesses that supply packed produce to consumers. This mini review considers the state of the art, as well as any developments required in robotic technology for the automation of inspection and packing of whole unprocessed fresh produce. There is a particular focus on robotic end effectors for the handling of fresh produce. We consider the role of soft robotics research in meeting hygiene and safety requirements, as well as the current limited range of end effectors for handling of highly varied and delicate produce types. Future directions are discussed based on the observation of available current technology available in research and the application to commercial practice.
{"title":"The adoption of robotics in pack houses for fresh produce handling","authors":"Barry J Mulholland, Pardeep S Panesar, Philip H Johnson","doi":"10.1080/14620316.2023.2247835","DOIUrl":"https://doi.org/10.1080/14620316.2023.2247835","url":null,"abstract":"Fresh produce handling, particularly in final inspection and pack, is highly dependent on dextrous human labour. As part of a relatively low-profit margin industry, rising wage costs and labour shortages are impacting the viability of this sector and are a direct threat to global food security. Adoption of robotics is required to automate delicate handling tasks; this is a key goal for sustainable and profitable businesses that supply packed produce to consumers. This mini review considers the state of the art, as well as any developments required in robotic technology for the automation of inspection and packing of whole unprocessed fresh produce. There is a particular focus on robotic end effectors for the handling of fresh produce. We consider the role of soft robotics research in meeting hygiene and safety requirements, as well as the current limited range of end effectors for handling of highly varied and delicate produce types. Future directions are discussed based on the observation of available current technology available in research and the application to commercial practice.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136357826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-10DOI: 10.1080/14620316.2023.2265360
Young Bok Yun, Hyeonju Jeong, Younsup Cho, Sunggil Kim
ABSTRACTYuzu (Citrus junos Sieb. Ex Tanaka) is widely cultivated in Asia. However, a system for variety identification has not been developed yet. RNA-seq analysis was performed to identify variants among five yuzu samples including a seedless variety. However, only four variants were found, suggesting that the seedless trait might originate from bud mutations. To identify more variants, whole-genome resequencing was performed. A total of 140 reliable variants were screened. Based on these variants, 30 high-resolution melting (HRM) markers were developed. After genotyping 265 yuzu samples using the HRM markers, the yuzu samples were classified into 18 types. Three yuzu-like samples (B-37-1, B-UK2, and B-MD6-8) showing different fruit morphologies and off-type HRM peak patterns were assumed to originate from interspecific hybridisation. To identify the parent species of these yuzu-like samples, nucleotide sequences of a hypervariable chloroplast genomic region and a nuclear CitRWP gene responsible for nucellar embryony were obtained. Chloroplast genome sequence analysis indicated that the maternal parents of B-37-1 and B-MD6-8 were yuzu, whereas the B-UK2 sequence was identical to that of sweet orange. Moreover, the CitRWP sequences indicated that the male parents of B-37-1, B-UK2, and B-MD6-8 were mandarin, yuzu, and pummelo, respectively.KEYWORDS: YuzuCitrus junosvariety identificationmolecular markerhigh-resolution meltingnucellar embryony AcknowledgementsThe authors thank Ji-hwa Heo, Jeong-An Yoo, and Su-jeong Kim for their dedicated technical assistance. This study was supported by the “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ016161)” funded by the Rural Development Administration, Republic of Korea, and the BK21 FOUR grant funded by the Ministry of Education of Korea and National Research Foundation of Korea.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementTranscriptome and whole genome resequencing data obtained in this study are accessible at NCBI SRA database.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2265360.Additional informationFundingThe work was supported by the Rural Development Administration [PJ016161].
{"title":"Analysis of genetic diversity of yuzu ( <i>Citrus junos</i> Sieb. Ex Tanaka) using single nucleotide polymorphisms identified through RNA-seq and whole-genome resequencing analyses","authors":"Young Bok Yun, Hyeonju Jeong, Younsup Cho, Sunggil Kim","doi":"10.1080/14620316.2023.2265360","DOIUrl":"https://doi.org/10.1080/14620316.2023.2265360","url":null,"abstract":"ABSTRACTYuzu (Citrus junos Sieb. Ex Tanaka) is widely cultivated in Asia. However, a system for variety identification has not been developed yet. RNA-seq analysis was performed to identify variants among five yuzu samples including a seedless variety. However, only four variants were found, suggesting that the seedless trait might originate from bud mutations. To identify more variants, whole-genome resequencing was performed. A total of 140 reliable variants were screened. Based on these variants, 30 high-resolution melting (HRM) markers were developed. After genotyping 265 yuzu samples using the HRM markers, the yuzu samples were classified into 18 types. Three yuzu-like samples (B-37-1, B-UK2, and B-MD6-8) showing different fruit morphologies and off-type HRM peak patterns were assumed to originate from interspecific hybridisation. To identify the parent species of these yuzu-like samples, nucleotide sequences of a hypervariable chloroplast genomic region and a nuclear CitRWP gene responsible for nucellar embryony were obtained. Chloroplast genome sequence analysis indicated that the maternal parents of B-37-1 and B-MD6-8 were yuzu, whereas the B-UK2 sequence was identical to that of sweet orange. Moreover, the CitRWP sequences indicated that the male parents of B-37-1, B-UK2, and B-MD6-8 were mandarin, yuzu, and pummelo, respectively.KEYWORDS: YuzuCitrus junosvariety identificationmolecular markerhigh-resolution meltingnucellar embryony AcknowledgementsThe authors thank Ji-hwa Heo, Jeong-An Yoo, and Su-jeong Kim for their dedicated technical assistance. This study was supported by the “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ016161)” funded by the Rural Development Administration, Republic of Korea, and the BK21 FOUR grant funded by the Ministry of Education of Korea and National Research Foundation of Korea.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementTranscriptome and whole genome resequencing data obtained in this study are accessible at NCBI SRA database.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2265360.Additional informationFundingThe work was supported by the Rural Development Administration [PJ016161].","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136295872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-09DOI: 10.1080/14620316.2023.2263757
Miao Huang, Jin Zhao
ABSTRACTPitaya fruit has highly edible, medicinal, and economic value. Currently, pitaya is often used as fresh fruit to meet the market demand of consumers. However, pitaya fruit is prone to mechanical damage, microbial infection, and softening and decay after harvest, affecting the quality and shelf life of fresh pitaya fruit. Therefore, many technologies have been developed to extend the postharvest storage time of fresh pitaya. This review summarises the internal and external factors that affect the deterioration in quality of pitaya fruit after harvest and introduces the types of preservation technologies for pitaya fruit presently available. This includes physical, chemical, and techniques based on natural substances to extend the shelf life of postharvest pitaya fruit, as well as the control methods of postharvest diseases and pests. Future research needs to consider the combined application of various technologies to improve the preservation of pitaya.KEYWORDS: PitayapostharvestTechnologypreservationqualityshelf life AcknowledgementsThis review is supported by Outstanding Young Scientist Program of Guizhou Province under grant number KY [2021]028; Regional Fund of the National Natural Science Foundation of China (32260799); Cultivation Research Program of Guizhou University under grant numbers [2019]42.Disclosure statementThe authors declare no conflict of interest.Data availability statementAll relevant data are within the paper. And more information can be found in the references.Additional informationFundingThis work was supported by the Regional Fund of the National Natural Science Foundation of China [32260799]; Cultivation Research Program of Guizhou University [[2019]42]; Outstanding Young Scientist Program of Guizhou Province [KY [2021]028].
{"title":"Recent advances in postharvest storage and preservation technology of pitaya (dragon fruit)","authors":"Miao Huang, Jin Zhao","doi":"10.1080/14620316.2023.2263757","DOIUrl":"https://doi.org/10.1080/14620316.2023.2263757","url":null,"abstract":"ABSTRACTPitaya fruit has highly edible, medicinal, and economic value. Currently, pitaya is often used as fresh fruit to meet the market demand of consumers. However, pitaya fruit is prone to mechanical damage, microbial infection, and softening and decay after harvest, affecting the quality and shelf life of fresh pitaya fruit. Therefore, many technologies have been developed to extend the postharvest storage time of fresh pitaya. This review summarises the internal and external factors that affect the deterioration in quality of pitaya fruit after harvest and introduces the types of preservation technologies for pitaya fruit presently available. This includes physical, chemical, and techniques based on natural substances to extend the shelf life of postharvest pitaya fruit, as well as the control methods of postharvest diseases and pests. Future research needs to consider the combined application of various technologies to improve the preservation of pitaya.KEYWORDS: PitayapostharvestTechnologypreservationqualityshelf life AcknowledgementsThis review is supported by Outstanding Young Scientist Program of Guizhou Province under grant number KY [2021]028; Regional Fund of the National Natural Science Foundation of China (32260799); Cultivation Research Program of Guizhou University under grant numbers [2019]42.Disclosure statementThe authors declare no conflict of interest.Data availability statementAll relevant data are within the paper. And more information can be found in the references.Additional informationFundingThis work was supported by the Regional Fund of the National Natural Science Foundation of China [32260799]; Cultivation Research Program of Guizhou University [[2019]42]; Outstanding Young Scientist Program of Guizhou Province [KY [2021]028].","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACTGuava (Psidium guajava L.), a member of the family Myrtaceae, is the third richest source of vitamin C after Barbados cherry and aonla. Guava has many medicinal properties associated with every plant part. Coloured guava has additional nutritive value due to its lycopene, anthocyanin, and carotenoid content, which impart colour to its pulp and serve as potential antioxidants. In this review, the various categories of pulp colour in guava, genetics of pulp colour, pigments responsible for various pulp colours, and the biosynthetic pathway of those pigments are presented in detail. The recent advances in guava show that both carotenoids and anthocyanins contribute significantly to its pulp colour. The final pulp colour of the guava depends on the relative proportion of the pigments, i.e. anthocyanins, lycopene, and other carotenoids.KEYWORDS: AnthocyaninsgeneticsGuavalycopenepsidium guajava Lpulp colour AcknowledgementsThis work is supported by ICAR-IARI, New Delhi, 110012, India.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementData presented in all the figures and tables of the review paper are compiled from various sources.
{"title":"Breeding for pulp colour in Guava: current status and opportunities","authors":"Hanamant Ankad, Akshay Dhillon, Madhubala Thakre, Rutuparna Senapati, Ram Kumar, G. Nayan Deepak, Nagaraja Arumugam, M.K. Verma, Gopala Krishnan S., Amitha Mithra","doi":"10.1080/14620316.2023.2251995","DOIUrl":"https://doi.org/10.1080/14620316.2023.2251995","url":null,"abstract":"ABSTRACTGuava (Psidium guajava L.), a member of the family Myrtaceae, is the third richest source of vitamin C after Barbados cherry and aonla. Guava has many medicinal properties associated with every plant part. Coloured guava has additional nutritive value due to its lycopene, anthocyanin, and carotenoid content, which impart colour to its pulp and serve as potential antioxidants. In this review, the various categories of pulp colour in guava, genetics of pulp colour, pigments responsible for various pulp colours, and the biosynthetic pathway of those pigments are presented in detail. The recent advances in guava show that both carotenoids and anthocyanins contribute significantly to its pulp colour. The final pulp colour of the guava depends on the relative proportion of the pigments, i.e. anthocyanins, lycopene, and other carotenoids.KEYWORDS: AnthocyaninsgeneticsGuavalycopenepsidium guajava Lpulp colour AcknowledgementsThis work is supported by ICAR-IARI, New Delhi, 110012, India.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementData presented in all the figures and tables of the review paper are compiled from various sources.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136313263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.1080/14620316.2023.2258879
Jonas V Müller
ABSTRACTCultivated pears are economically one of the most important fruits in temperate regions. Two separate taxonomic groups are distinguished, a European and Southwest Asian group, and an East Asian group. Pears have been cultivated at least since Ancient Greek times. Their taxonomy is often confusing, with 20 to 30 widely recognised species. Their outbreeding character and widespread hybridisation led to a huge number of different forms and crosses. The Caucasus is regarded as one of two main centres of diversity in the European and Southwest Asian group. In the Republic of Georgia, several wild species incl. Pyrus communis subsp. caucasica occur. Pyrus communis subsp. caucasica is the sister taxa of the European wild pear Pyrus communis subsp. pyraster, which can morphologically not be distinguished. The natural ranges of these two sister taxa do not overlap. Scientists assume two separate domestication events, in Europe and in Caucasus. In the Caucasus region, local pear cultivars remain genetically very close to wild pears which were used as rootstock, while the fruits are still used to produce a local schnapps. Today, wild pears are often endangered due to habitat loss and fragmentation. Wild pears constitute a rich genetic basis for future breeding.KEYWORDS: Pearscultivationplant breedingconservationCaucasuslandraces AcknowledgementsI would like to thank two anonymous reviewers for their comments which helped improve this review article. I would like to thank David Kikodze, Shalva Sikharulidze, and Zezva Asanidze (Ilia State University, Tbilisi, Georgia) for their valuable information on the distribution and use of wild pears in Georgia. David Kikodze and Filippo Guzzon commented on an earlier version of this manuscript. Stéphane Rivière produced the maps shown as Figures 5 and 6. This work did not receive external funding.Disclosure statementNo potential conflict of interest was reported by the author.Data availability statementThe author confirms that no additional data are available.
摘要栽培梨是温带地区最重要的经济水果之一。两个独立的分类群被区分开来，一个是欧洲和西南亚群，一个是东亚群。至少从古希腊时代起，人们就开始种植梨了。它们的分类常常令人困惑，只有20到30种被广泛认可的物种。它们的近亲繁殖特征和广泛的杂交导致了大量不同的形式和杂交。高加索地区被认为是欧洲和西南亚群体中两个主要的多样性中心之一。在格鲁吉亚共和国，几种野生种包括Pyrus communis subsp.。caucasica发生。梨属植物。高加索是欧洲野生梨(Pyrus communis)亚种的姐妹分类群。Pyraster，在形态上无法区分。这两个姊妹分类群的自然分布范围不重叠。科学家们假设在欧洲和高加索地区发生了两次不同的驯化事件。在高加索地区，当地的梨品种在遗传上与用作砧木的野生梨非常接近，而其果实仍然用于生产当地的杜松子酒。如今，由于栖息地的丧失和碎片化，野生梨经常濒临灭绝。野生梨为今后的育种提供了丰富的遗传基础。关键词:梨栽培;植物育种;保护;高加索地区;我要感谢David Kikodze、Shalva Sikharulidze和Zezva Asanidze(格鲁吉亚第比利斯伊利亚州立大学)就格鲁吉亚野生梨的分布和利用提供的宝贵信息。David Kikodze和Filippo Guzzon对这份手稿的早期版本进行了评论。stenjophane rivire制作了如图5和图6所示的地图。这项工作没有得到外部资助。披露声明作者未报告潜在的利益冲突。数据可用性声明作者确认没有其他数据可用。
{"title":"Domestication of wild pears in Europe, with specific emphasis on the Caucasian endemic pear <i>Pyrus communis</i> subsp. <i>caucasica</i> (Fed.) Browicz","authors":"Jonas V Müller","doi":"10.1080/14620316.2023.2258879","DOIUrl":"https://doi.org/10.1080/14620316.2023.2258879","url":null,"abstract":"ABSTRACTCultivated pears are economically one of the most important fruits in temperate regions. Two separate taxonomic groups are distinguished, a European and Southwest Asian group, and an East Asian group. Pears have been cultivated at least since Ancient Greek times. Their taxonomy is often confusing, with 20 to 30 widely recognised species. Their outbreeding character and widespread hybridisation led to a huge number of different forms and crosses. The Caucasus is regarded as one of two main centres of diversity in the European and Southwest Asian group. In the Republic of Georgia, several wild species incl. Pyrus communis subsp. caucasica occur. Pyrus communis subsp. caucasica is the sister taxa of the European wild pear Pyrus communis subsp. pyraster, which can morphologically not be distinguished. The natural ranges of these two sister taxa do not overlap. Scientists assume two separate domestication events, in Europe and in Caucasus. In the Caucasus region, local pear cultivars remain genetically very close to wild pears which were used as rootstock, while the fruits are still used to produce a local schnapps. Today, wild pears are often endangered due to habitat loss and fragmentation. Wild pears constitute a rich genetic basis for future breeding.KEYWORDS: Pearscultivationplant breedingconservationCaucasuslandraces AcknowledgementsI would like to thank two anonymous reviewers for their comments which helped improve this review article. I would like to thank David Kikodze, Shalva Sikharulidze, and Zezva Asanidze (Ilia State University, Tbilisi, Georgia) for their valuable information on the distribution and use of wild pears in Georgia. David Kikodze and Filippo Guzzon commented on an earlier version of this manuscript. Stéphane Rivière produced the maps shown as Figures 5 and 6. This work did not receive external funding.Disclosure statementNo potential conflict of interest was reported by the author.Data availability statementThe author confirms that no additional data are available.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135060659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：