Design of microemulsion formulations loaded Scutellaria salviifolia Benth, Sideritis libanotica Labill. subsp. linearis (Bentham) Bornm, and Ziziphora clinopodioides Lam. extracts from Turkey and in vitro evaluation of their biological activities
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引用次数: 4
Abstract
: The species belonging to the Lamiaceae family have been useful tools for many years as a folk remedy. In the present study, a microemulsion system was developed with extract of Scutellaria salviifolia Benth (endemic), Sideritis libanotica Labill. subsp. linearis (Bentham) Bornm (endemic), and Ziziphora clinopodioides Lam. Both and related microemulsion formulations were also investigated for their potential biological activities. Pseudo-ternary phase diagrams of formulations were constructed consisting of oil/ mixed surfactant/water emulsion system and a quaternary component. Antioxidant activity was determined by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. For antimicrobial activity determination, on the other hand, microdilution method was performed with Escherichia coli 25922, Staphylococcus aureus 29213, Candida albicans 14053, and Candida krusei 6258 standard strains. Tested extracts showed S. aureus-specific antibacterial activity with MIC values 64 µg/mL, 256 µg/mL, and 512 µg/mL for S. libanotica subsp. linearis, S. salviifolia, and Z. clinopodioides, respectively. For any other tested strain, considerable antimicrobial activity was not observed (MIC values > 1024 µg/mL). Related formulations were not superior to extracts in terms of antimicrobial activity. All extracts and related formulations appear to have antioxidant activity, and the antioxidant capacity of all formulations is higher than the extracts. When compared to ascorbic acid, formulation of S. libanotica subsp. linearis extract has stronger antioxidant activity. Sideritis libanotica subsp. linearis extract had a remarkable antibacterial activity against S. aureus. It also had a stronger antioxidant capacity than ascorbic acid. Besides, all formulations developed from extracts had antioxidant activity enhancing properties, although they did not contribute to antimicrobial activity. In conclusion, considering the potential antiinflammatory activity due to antioxidant activity, it is predicted that especially the formulation developed with Sideritis libanotica subsp. linearis extract is worth exploring with further studies for topical treatment of S. aureus-infected wounds and burns.
期刊介绍：
The Turkish Journal of Botany is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts manuscripts (in English) covering all areas of plant biology (including genetics, evolution, systematics, structure, function, development, diversity, conservation biology, biogeography, paleobotany, ontogeny, functional morphology, ecology, reproductive biology, and pollination biology), all levels of organisation (molecular to ecosystem), and all plant groups and allied organisms (algae, fungi, and lichens). Authors are required to frame their research questions and discuss their results in terms of major questions in plant biology. In general, papers that are too narrowly focused, purely descriptive, or broad surveys, or that contain only preliminary data or natural history, will not be considered (*).
The following types of article will be considered:
1. Research articles: Original research in various fields of botany will be evaluated as research articles.
2. Research notes: These include articles such as preliminary notes on a study or manuscripts on the morphological, anatomical, cytological, physiological, biochemical, and other properties of plant, algae, lichen and fungi species.
3. Reviews: Reviews of recent developments, improvements, discoveries, and ideas in various fields of botany.
4. Letters to the editor: These include opinions, comments relating to the publishing policy of the Turkish Journal of Botany, news, and suggestions. Letters should not exceed one journal page.
(*) 1. Raw floristic lists (of algae, lichens, fungi, or plants), species descriptions, chorological studies, and plant sociology studies without any additional independent approaches.
2. Comparative morphology and anatomy studies (that do not cover a family, tribe, subtribe, genus, subgenus, section, subsection, or species complexes with taxonomical problems) without one or more independent additional approaches such as phylogenetical, micromorphological, chromosomal and anatomical analyses.
3. Revisions of family, tribe, genus, subgenus, section, subsection, or species complexes without any original outputs such as taxonomical status changes, IUCN categories, and phenological and ecological analyses.
4. New taxa of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group.
New records of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 3 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms.
5. New taxa of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group.
New records of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 5 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms.
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