Effect of sevoflurane postconditioning on myocardial oncosis and autophagy in ischemia-reperfusion injury in isolated rat heart

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY Bangladesh Journal of Pharmacology Pub Date : 2018-08-28 DOI:10.3329/BJP.V13I3.36437

Q. Tang, Yang Xie, Hong Xie, Wenqiang Jiang, Z. Fang

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Abstract

The effects of sevoflurane postconditioning on myocardial oncosis and autophagy were studied in 32 isolated rat heart of ischemia-reperfusion injury. The hearts were perfused by: a) Sham surgery group, b) simple sevoflurane group, c) simple ischemia-reperfusion group (I/R) group, and d) sevoflurane postconditioning group. The ratios between the gray density values of the target bands to the gray density value of GADPH were used to reflect the Beclin-1, LC3II/I, and porimin expression levels. The LC3II/I level in the sevoflurane postconditioning group was lower than the level in the I/R group. The porimin level in the sevoflurane postconditioning group was lower than the level in the I/R group. The myocardial infarction range in the sevoflurane postconditioning group (33 ± 5%) was significantly diminished compared with the range in the I/R group (53 ± 6%) (p<0.05). Sevoflurane decreased the occurrence of oncosis and alleviated myocardial ischemia-reperfusion injury by inhibiting MPTP opening.

Video Clip of Methodology:

14 min 31 sec Full Screen Alternate

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七氟醚后处理对离体大鼠心脏缺血再灌注损伤心肌细胞膨胀和自噬的影响

研究了七氟醚后处理对32例离体大鼠心肌缺血再灌注损伤后心肌膨胀和自噬的影响。心脏灌注采用：a）假手术组，b）单纯七氟醚组，c）单纯缺血再灌注组（I/R），d）七氟醚后处理组。靶带的灰度密度值与GADPH的灰度密度之间的比率用于反映Beclin-1、LC3II/I和porimin的表达水平。七氟醚后处理组的LC3II/I水平低于I/R组。七氟醚后处理组的porimin水平低于I/R组。七氟醚后处理组的心肌梗死范围（33±5%）与I/R组的心肌梗塞范围（53±6%）相比显著缩小（方法学视频剪辑：14分31秒全屏交替

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.