The ameliorative effect of Lactobacillus paracasei BEJ01 against FB1 induced spermatogenesis disturbance, testicular oxidative stress and histopathological damage.

IF 2.8 4区医学 Q2 TOXICOLOGY Toxicology Mechanisms and Methods Pub Date : 2022-06-21 DOI:10.1080/15376516.2022.2087049

Khawla Ezdini, Jalila Ben Salah-Abbès, Hela Belgacem, Bolanle Ojokoh, Kamel Chaieb, Samir Abbès

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Abstract

Fumonisin B1 (FB1) is a possible carcinogenic molecule for humans as classified by the International Agency for Research on Cancer (IARC) in 2B group. In livestock, it is responsible for several mycotoxicoses and economic losses. Lactobacillus strains, inhabitants of a wide range of foodstuffs and the gastrointestinal tract, are generally recognized as safe (GRAS). Thus, the aim of this work was to evaluate the protective effect of Lactobacillus paracasei (LP) against FB1-induced reprotoxicities including testicular histopathology, sperm quality disturbance, and testosterone level reduction.Pubescent mice were divided randomly into four groups and treated for 10 days. Group 1: Control; Group 2: FB1 (100 μg/kg b.w); Group 3: LP (2 × 10⁹ CFU/kg b.w); Group 4: LP (2 × 10⁹ CFU/kg b.w) and FB1 (100 μg/kg b.w). After the end of the treatment, animals were sacrificed. Plasma, epididymis, and testis were collected for reproductive system studies.Our results showed that FB1 altered epididymal sperm quality, generated oxidative stress, and induced histological alterations. Interestingly, these deleterious effects have been counteracted by the LP administration in mice.In conclusion, LP was able to prevent FB1-reproductive system damage in BALB/c mice and could be validated as an anti-caking agent in an animal FB1-contaminated diet.

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副干酪乳杆菌BEJ01对FB1诱导的精子发生障碍、睾丸氧化应激和组织病理学损伤的改善作用

摘要伏马菌素B1（FB1）是一种可能的人类致癌分子，被国际癌症研究机构（IARC）分为2B组。在牲畜中，它对几种真菌中毒和经济损失负有责任。乳酸杆菌菌株广泛存在于各种食品和胃肠道中，通常被认为是安全的（GRAS）。因此，本工作的目的是评估副干酪乳杆菌（LP）对FB1诱导的再毒性的保护作用，包括睾丸组织病理学、精子质量紊乱和睾酮水平降低。将Publich小鼠随机分为四组，治疗10 天。第1组：对照组；第2组：FB1（100 μg/kg b.w）；第3组：LP（2 × 109 CFU/kg b.w）；第4组：LP（2 × 109 CFU/kg b.w）和FB1（100 μg/kg体重）。治疗结束后，处死动物。采集血浆、附睾和睾丸进行生殖系统研究。我们的研究结果表明，FB1改变了附睾精子的质量，产生了氧化应激，并诱导了组织学改变。有趣的是，这些有害影响已经被小鼠服用LP所抵消。总之，LP能够预防BALB/c小鼠的FB1生殖系统损伤，并且可以在受FB1污染的动物饮食中被验证为抗结块剂。

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Toxicology Mechanisms and Methods TOXICOLOGY-

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3.10%

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期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.