Influence of hydrodynamic cavitation on functional, nutritional, and structural characteristics of egg-white protein hydrolysates

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-08-22 DOI:10.1016/j.fhfh.2023.100153
Niveditha Asaithambi, Poonam Singha, Sushil Kumar Singh
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Abstract

The present work demonstrates the effect of hydrodynamic cavitation (HC) (2 mm Orifice plate) as a pretreatment for egg white protein hydrolysates (EWPH) production. The obtained EWPH was evaluated for various physiochemical (degree of hydrolysis), functional (emulsifying, foaming), structural and nutritional properties (antioxidant activity and in-vitro digestibility). The egg white solutions (5% solid content) were pretreated for 10, 15 and 20 min with HC and later hydrolyzed using papain enzyme for 90 min. The structural analysis revealed that HC unfolded the protein structure which was confirmed through the formation of β-sheet (from 15 to 46%) and loss of α-helix (34 to 14%) content with increasing treatment time. Through the exposure of hydrophobic bonds, the degree of hydrolysis and surface hydrophobicity increased, which eventually improved the nutritional and functional properties of EWPH. The HC-15 min treated samples had the highest zeta potential (-25.4 mV) with the lowest average particle size (346.5 nm) and denaturation temperature (70.67°C). Further increase in treatment time led to instability of hydrolysates. HC effectively improved the functional and nutritional properties of EWPH and a treatment time of 15 min is recommended for obtaining EWPH with improved properties.

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水力空化对蛋清蛋白水解物功能、营养和结构特性的影响
本研究证明了水动力空化(HC) (2mm孔板)作为蛋白水解物(EWPH)生产的预处理效果。对所得EWPH进行了各种理化(水解程度)、功能(乳化、发泡)、结构和营养特性(抗氧化活性和体外消化率)的评价。将固含量为5%的蛋清溶液分别用HC预处理10、15和20 min,然后用木瓜蛋白酶水解90 min。结构分析表明,随着处理时间的增加,HC使蛋白结构展开,通过β-片的形成(从15%到46%)和α-螺旋含量的损失(从34%到14%)证实了这一点。通过疏水键的暴露,提高了水解程度和表面疏水性,最终改善了EWPH的营养和功能性能。HC-15 min处理的样品zeta电位最高(-25.4 mV),平均粒径最小(346.5 nm),变性温度最低(70.67℃)。进一步增加处理时间导致水解产物不稳定。HC有效地改善了EWPH的功能和营养特性,建议处理时间为15 min,以获得性能改善的EWPH。
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4.50
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审稿时长
61 days
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