Profiling of Microbial Community in Rearing Water of White Shrimp (Litopenaeus vannamei) Infected with White Feces Disease Syndrome

K. Rahardjo, Woro Hastuti Setyantini, M. Amin
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Abstract

Shrimp farmers have reported mass mortality of white shrimp (Litopenaeus vannamei), reaching 2-3 kg/day in Gerongan Village, Kraton District, Pasuruan Regency in mid-2021. Preliminary analysis suggests that mass mortality was caused by bacterial diseases. Thus, to find out the main pathogen causing the mass mortality, the present study investigated microbial composition in rearing media of white shrimp (Litopenaeus vannamei) infected by the white feces disease (WFD) and healthy white shrimp using next-generation sequencing (NGS) technology. The research was conducted by collecting normal water samples and infected shrimp pond water samples. The results of the NGS assay showed that Vibrio vulnificus was found dominant in WFD-infected shrimp pond water, therefore was suspected to be the main cause of WFD.
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感染白粪病综合征的凡纳滨对虾养殖水体微生物群落特征
据虾农报告,2021年年中,帕苏鲁安县克拉顿区Gerongan村的白虾(凡纳滨对虾)大量死亡,达到每天2-3公斤。初步分析表明,大规模死亡是由细菌性疾病引起的。因此,为了找出导致大规模死亡的主要病原体,本研究采用下一代测序技术对感染白粪病(WFD)的白对虾(Litopenaeus vannamei)和健康白对虾饲养基中的微生物组成进行了研究。本研究是通过采集正常水体和受感染的虾池水体样本进行的。NGS检测结果表明,创伤弧菌在感染WFD的虾池水中占优势,因此被怀疑是WFD的主要原因。
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审稿时长
6 weeks
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