Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.43013
Diana Maulianawati, Hendri Kiing, Dena Pramita Dewi, Heni Irawati, Muhammad Amien
Applying biofloc technology in the intensive and extensive culture of Clarias gariepinus can improve water quality and be used to feed fish. Aeration systems were a critical unit supporting biofloc and water quality. This study's objective was assessment to various types of diffusers on the growth and water quality in a C. gariepinus culture. Two types of diffusers unit were prepared for the experiment, there are air tube diffuser (AT) and air stone diffuser (AS). Growth parameters, water quality, and volume of biofloc were observed within 30 days. The survival rate, weight gain, average body weight, and specific growth rate of C. gariepinus were higher in the tanks that used air tubes (98%, 485.29 %, 7.52 g, 5.89%) than in the tanks that used air stones (92 %, 385.94 %, 5.98 g, 5.23%). The volume of biofloc range from 5.40-18.80 ml/L in AT tanks and 4.60-14.00 ml/L in AS tanks. There is no significant difference (p > 0.05) in water quality parameters and FCR value. However, using the air tube diffuser showed better results with the growth performance, survival rate, and formation of biofloc.
{"title":"The Application of Different Types of Diffusers for African Catfish (Clarias gariepinus) Culture in Biofloc Systems: Effects on Growth and Water Quality","authors":"Diana Maulianawati, Hendri Kiing, Dena Pramita Dewi, Heni Irawati, Muhammad Amien","doi":"10.20473/jafh.v13i1.43013","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.43013","url":null,"abstract":"Applying biofloc technology in the intensive and extensive culture of Clarias gariepinus can improve water quality and be used to feed fish. Aeration systems were a critical unit supporting biofloc and water quality. This study's objective was assessment to various types of diffusers on the growth and water quality in a C. gariepinus culture. Two types of diffusers unit were prepared for the experiment, there are air tube diffuser (AT) and air stone diffuser (AS). Growth parameters, water quality, and volume of biofloc were observed within 30 days. The survival rate, weight gain, average body weight, and specific growth rate of C. gariepinus were higher in the tanks that used air tubes (98%, 485.29 %, 7.52 g, 5.89%) than in the tanks that used air stones (92 %, 385.94 %, 5.98 g, 5.23%). The volume of biofloc range from 5.40-18.80 ml/L in AT tanks and 4.60-14.00 ml/L in AS tanks. There is no significant difference (p > 0.05) in water quality parameters and FCR value. However, using the air tube diffuser showed better results with the growth performance, survival rate, and formation of biofloc. ","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.36213
Elisabeth Tirani, Maftuch Maftuch, Mohammad Fadjar, Muhammad Awaluddin
Non-specific defense is the main defense in fish. One of the natural ingredients as a source immunostimulant isH. illucens larvae with a protein content of up to 30%. H. illucens larvae contains alkaloids, tannins, terpenoidsand saponins as immunostimulating agents. Immunostimulants are biological compounds that can boost theimmune system body. The purpose of this study is to find out the effect of H. illucens larvae in increasingimmunity non specific in tilapia (O. niloticus) which infected with E. tarda. In giving H. illucens larvae feed adlibitum with concentrations of A(30%), B(40%), C(50%), K(0%). In this research method, a completelyrandomized design (CRD) was used with 5 treatments and 3 replications in which each aquarium contained 10tilapia. Parameters tested are leukocytes, differential leukocytes, erythrocytes and hemoglobin. The resultsshowed that intake of 50% H. illucens larvae on tilapia could increase non-specific immunity, such as totalleukocytes, total erythrocytes, differential leukocytes, hemoglobin
{"title":"Effect of Hermetia illucens Larvae on the Hematology of Tilapia (Oreochromis niloticus) Infected Edwardsiella tarda","authors":"Elisabeth Tirani, Maftuch Maftuch, Mohammad Fadjar, Muhammad Awaluddin","doi":"10.20473/jafh.v13i1.36213","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.36213","url":null,"abstract":"Non-specific defense is the main defense in fish. One of the natural ingredients as a source immunostimulant isH. illucens larvae with a protein content of up to 30%. H. illucens larvae contains alkaloids, tannins, terpenoidsand saponins as immunostimulating agents. Immunostimulants are biological compounds that can boost theimmune system body. The purpose of this study is to find out the effect of H. illucens larvae in increasingimmunity non specific in tilapia (O. niloticus) which infected with E. tarda. In giving H. illucens larvae feed adlibitum with concentrations of A(30%), B(40%), C(50%), K(0%). In this research method, a completelyrandomized design (CRD) was used with 5 treatments and 3 replications in which each aquarium contained 10tilapia. Parameters tested are leukocytes, differential leukocytes, erythrocytes and hemoglobin. The resultsshowed that intake of 50% H. illucens larvae on tilapia could increase non-specific immunity, such as totalleukocytes, total erythrocytes, differential leukocytes, hemoglobin","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"523 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140417494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.40769
S. Arsad, Rut Suharni P Sihombing, Mohammad Mahmudi, O. M. Luthfi, Ikha Safitri, F. D. Pratiwi
Microalgae, micro-sized plant organisms, play a crucial role in aquatic ecosystems. While many microalgae inhabit substrates or the bottom of water bodies, several types are planktonic. This study aimed to identify the types and abundance of microalgae in both sediment and water column habitats, as well as to analyse the environmental factors influencing their abundance. The research encompasses observations of water quality factors, microalgae abundance, relative abundance, diversity index, evenness index, and dominance index. Statistical analyses were using non-metric multidimensional scaling (NMDS) and Canonical Correspondence Analysis (CCA). The study was conducted in May-June 2022, with bi-weekly sampling at three points within each location for two months. Microalgae identified in the coastal area of Probolinggo belong to the Bacillariophyceae, Cyanophyceae, and Chlorophyceae classes. The highest microalgae abundance in sediment habitat was 58,472 ind/cm2, while in the water column was 4,118 ind/l. Diversity, evenness, and dominance indices in both sediment and water column habitats ranged from 1.93 to 2.61, 0.88 to 0.98, and 0.09 to 0.10, respectively. NMDS and CCA analyses indicate a graphical representation of the Bacillariophyceae class, demonstrating its prevalence across all sites.
{"title":"Benthic and Planktonic Microalgae Community in Probolinggo Beach","authors":"S. Arsad, Rut Suharni P Sihombing, Mohammad Mahmudi, O. M. Luthfi, Ikha Safitri, F. D. Pratiwi","doi":"10.20473/jafh.v13i1.40769","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.40769","url":null,"abstract":"Microalgae, micro-sized plant organisms, play a crucial role in aquatic ecosystems. While many microalgae inhabit substrates or the bottom of water bodies, several types are planktonic. This study aimed to identify the types and abundance of microalgae in both sediment and water column habitats, as well as to analyse the environmental factors influencing their abundance. The research encompasses observations of water quality factors, microalgae abundance, relative abundance, diversity index, evenness index, and dominance index. Statistical analyses were using non-metric multidimensional scaling (NMDS) and Canonical Correspondence Analysis (CCA). The study was conducted in May-June 2022, with bi-weekly sampling at three points within each location for two months. Microalgae identified in the coastal area of Probolinggo belong to the Bacillariophyceae, Cyanophyceae, and Chlorophyceae classes. The highest microalgae abundance in sediment habitat was 58,472 ind/cm2, while in the water column was 4,118 ind/l. Diversity, evenness, and dominance indices in both sediment and water column habitats ranged from 1.93 to 2.61, 0.88 to 0.98, and 0.09 to 0.10, respectively. NMDS and CCA analyses indicate a graphical representation of the Bacillariophyceae class, demonstrating its prevalence across all sites.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.42197
Adelia Elviantari, Adi Suriyadin, Muhammad Haikal Abdurachman
Spiny lobster (Panulirus homarus) is a fishery commodity with high economic value. The classic problem that has not been resolved so far is the low survival rate of around 20-30% and an average growth rate of 180-230 grams. This can be influenced by nutritional factors, environment, stress and pathogen infection. This study aimed to identify pathogenic bacteria found in wild caught lobsters (Labangka) and cultivated lobsters (Bungin Island). The stages of this research began with taking samples in the field, followed by isolation and purification of bacteria (tail organs, gills, and hepatopancreas), morphological characteristics, and physiological tests of bacterial isolates. From the results of the study, it was found that the isolates grown on TSA media, showed a higher diversity of bacteria in natural lobsters compared to cultivated lobsters, this is what makes natural lobsters have a high survival rate because the diversity of microflora forms a symbiotic mutualism. Meanwhile, if we look at the diversity of pathogenic bacteria (Vibrio spp.), namely isolates grown on TCBS media, it shows that cultivated lobsters have more diverse pathogenic bacteria, namely three types of Vibrio (V. alginilyticus, V. Harvey and V. Parahemolyticus) are indicated, only natural lobsters identified V. alginilyticus.
{"title":"Identification of Vibrio spp. in Spiny Lobster (Panulirus homarus) from Natural Catch and Culture In Batu Bangka Village, Sumbawa","authors":"Adelia Elviantari, Adi Suriyadin, Muhammad Haikal Abdurachman","doi":"10.20473/jafh.v13i1.42197","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.42197","url":null,"abstract":"Spiny lobster (Panulirus homarus) is a fishery commodity with high economic value. The classic problem that has not been resolved so far is the low survival rate of around 20-30% and an average growth rate of 180-230 grams. This can be influenced by nutritional factors, environment, stress and pathogen infection. This study aimed to identify pathogenic bacteria found in wild caught lobsters (Labangka) and cultivated lobsters (Bungin Island). The stages of this research began with taking samples in the field, followed by isolation and purification of bacteria (tail organs, gills, and hepatopancreas), morphological characteristics, and physiological tests of bacterial isolates. From the results of the study, it was found that the isolates grown on TSA media, showed a higher diversity of bacteria in natural lobsters compared to cultivated lobsters, this is what makes natural lobsters have a high survival rate because the diversity of microflora forms a symbiotic mutualism. Meanwhile, if we look at the diversity of pathogenic bacteria (Vibrio spp.), namely isolates grown on TCBS media, it shows that cultivated lobsters have more diverse pathogenic bacteria, namely three types of Vibrio (V. alginilyticus, V. Harvey and V. Parahemolyticus) are indicated, only natural lobsters identified V. alginilyticus.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"70 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.49072
Ashari Fahrurrozi, B. D. Madusari, M. B. Syakirin, L. Linayati, Tri Yusufi Mardiana, Heri Ariadi Wijianto, Budianto Budianto
The flood disaster in the coastal area of Pekalongan City resulted in stagnant water, thus impacting the livelihoods of the community previously. The adaptation that has been made to deal with these conditions is by cultivating saline tilapia. Feed is the most important factor in supporting the growth of saline tilapia production. However, research on feed quality and frequency that has been carried out for tilapia production has not been optimal in obtaining the results obtained. This study aims to determine the effect of different feeding rates on growth performance, feed utilization and survival rate of tilapia reared in stagnant brackish water. The study consisted of feeding rates of 3%, 5%, 7% and 9% with three replications, and parameters were observed every seven days. Based on the research results it is known that the optimal feeding rate in saline tilapia culture ranges from 6.67% - 7% based on growth parameters. Although the other parameters do not show the same feeding rate as the growth parameter, these parameters are still in a good range for saline tilapia culture.
{"title":"Optimization Feeding Rate Towards Saline Tilapia Production in Stagnant Waters, Pekalongan City","authors":"Ashari Fahrurrozi, B. D. Madusari, M. B. Syakirin, L. Linayati, Tri Yusufi Mardiana, Heri Ariadi Wijianto, Budianto Budianto","doi":"10.20473/jafh.v13i1.49072","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.49072","url":null,"abstract":"The flood disaster in the coastal area of Pekalongan City resulted in stagnant water, thus impacting the livelihoods of the community previously. The adaptation that has been made to deal with these conditions is by cultivating saline tilapia. Feed is the most important factor in supporting the growth of saline tilapia production. However, research on feed quality and frequency that has been carried out for tilapia production has not been optimal in obtaining the results obtained. This study aims to determine the effect of different feeding rates on growth performance, feed utilization and survival rate of tilapia reared in stagnant brackish water. The study consisted of feeding rates of 3%, 5%, 7% and 9% with three replications, and parameters were observed every seven days. Based on the research results it is known that the optimal feeding rate in saline tilapia culture ranges from 6.67% - 7% based on growth parameters. Although the other parameters do not show the same feeding rate as the growth parameter, these parameters are still in a good range for saline tilapia culture.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140419993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The demand for common carp in Indonesia is high, and the success of seed production relies on the quality of the broodstock. However, freshwater aquaculture faces challenges from diseases, particularly parasites like Argulus japonicus. This study aimed to determine the prevalence, intensity, and degree of infestation of A. japonicus on common carp. The research method was survey. The research was conducted in October 2019 at Ngawi Fish Hatchery Center and in November 2023 at Tambakrejo Village, Waru, Sidoarjo, East Java, Indonesia. There were 26 broodstock of common carp as samples. A. japonicus were ectoparasites found to infest 10 common carp (Cyprinus carpio) broodstock that were ready to spawn at Fish Hatchery Center in Ngawi, East Java and 16 common carp (C. carpio) that raised from Tambakrejo Village farmer in Waru, Sidoarjo, East Java. Prevalence of the fish infested with A. japonicus was same in 100% at both regions. However, there is different in the intensity of A. japonicus at Ngawi Fish Hatchery Center (29 individuals/head) and at Tambakrejo Village, Waru, Sidoarjo (42,5 individuals/head). But, the degree of infestation in both location was classified as severe. Attachment site of A. japonicus were in the fish body surface, fins, and mouth. During the research, water quality is in normal limits that can be observed by 28°C temperature, 7.1 pH, and 2.6 mg/L DO. In conclusion, common carp in Ngawi Fish Hatchery Center and Tambakrejo Village, Waru, Sidoarjo, East Java, were infested with the heavy category of A. japonicus.
{"title":"Prevalence, Intensity and Degree of Infestation Profile Argulus japonicus Ectoparasite in Common Carp (Cyprinus carpio) at Ngawi and Tambakrejo, Sidoarjo, East Java, Indonesia","authors":"Sadida Anindya Bahtiar, Kismiyati, Nirattisai Petchsupa","doi":"10.20473/jafh.v13i1.54102","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.54102","url":null,"abstract":"The demand for common carp in Indonesia is high, and the success of seed production relies on the quality of the broodstock. However, freshwater aquaculture faces challenges from diseases, particularly parasites like Argulus japonicus. This study aimed to determine the prevalence, intensity, and degree of infestation of A. japonicus on common carp. The research method was survey. The research was conducted in October 2019 at Ngawi Fish Hatchery Center and in November 2023 at Tambakrejo Village, Waru, Sidoarjo, East Java, Indonesia. There were 26 broodstock of common carp as samples. A. japonicus were ectoparasites found to infest 10 common carp (Cyprinus carpio) broodstock that were ready to spawn at Fish Hatchery Center in Ngawi, East Java and 16 common carp (C. carpio) that raised from Tambakrejo Village farmer in Waru, Sidoarjo, East Java. Prevalence of the fish infested with A. japonicus was same in 100% at both regions. However, there is different in the intensity of A. japonicus at Ngawi Fish Hatchery Center (29 individuals/head) and at Tambakrejo Village, Waru, Sidoarjo (42,5 individuals/head). But, the degree of infestation in both location was classified as severe. Attachment site of A. japonicus were in the fish body surface, fins, and mouth. During the research, water quality is in normal limits that can be observed by 28°C temperature, 7.1 pH, and 2.6 mg/L DO. In conclusion, common carp in Ngawi Fish Hatchery Center and Tambakrejo Village, Waru, Sidoarjo, East Java, were infested with the heavy category of A. japonicus.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"25 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140419691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.45431
Prama Hartami, Eva Ayuzar, Salamah Salamah, Lilis Nurjannah, O. Carman, A. Alimuddin, Muhammad Rafi, Muhammad Fakhri
Motile Aeromonas Septicemia (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. This study aimed to test the resistance of triploid striped catfish to MAS infection before being released to farmers as a new commodity to increase national striped catfish production. The test was carried out for two months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila into triploid and diploid striped catfish. The challenge test was carried out by injecting the cultured A. hydrophila with a density of 107 cfu.mL-1 as much as 0.1 mL.ind-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 individuals measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood description were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at a 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila were gram-negative, oxidase and catalase positive, motile, could fermentatively convert sugar, and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid striped catfish are more resistant to infection with A. hydrophila than diploid.
由嗜水气单胞菌（Aeromonas hydrophila）引起的气单胞菌败血症（MAS）是一种常见的病原体，可侵袭淡水鱼类商品，包括作为带鱼新品系的三倍体带鱼。本研究旨在测试三倍体带鱼对 MAS 感染的抵抗力，然后将其作为新商品投放给养殖户，以提高全国带鱼产量。试验在梳邦苏卡曼迪鱼类育种研究所进行，为期两个月，向三倍体和二倍体带鱼注射嗜水青虫。在所有处理中，将密度为 107 cfu.mL-1 的嗜水蝇以 0.1 mL.ind-1 的剂量注射到鱼的肌肉中，而在对照组（-）处理中，则以 0.1 mL PBS 溶液注射到鱼的尾部。使用 Microsoft Excel 2010 和 SPSS 16.0 版软件对生存参数数据和血液描述进行定量分析，并进行方差分析（ANOVA），置信区间为 95%。生化检测重新定性的结果表明，嗜水青虫为革兰氏阴性，氧化酶和过氧化氢酶阳性，能运动，能发酵转化糖，并能在血液培养基中生长。试验后三倍体带鱼的存活率为 95%，第 7 天的红细胞总数和第 1 天的白细胞总数与二倍体带鱼有显著差异。因此，可以得出结论，三倍体条纹鲶比二倍体条纹鲶更能抵抗嗜水青虫的感染。
{"title":"Motile Aeromonas Septicemia (MAS) Disease Resistance Test by Aeromonas hydrophila on Triploid Striped Catfish (Pangasianodon hypopthalmus)","authors":"Prama Hartami, Eva Ayuzar, Salamah Salamah, Lilis Nurjannah, O. Carman, A. Alimuddin, Muhammad Rafi, Muhammad Fakhri","doi":"10.20473/jafh.v13i1.45431","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.45431","url":null,"abstract":"Motile Aeromonas Septicemia (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. This study aimed to test the resistance of triploid striped catfish to MAS infection before being released to farmers as a new commodity to increase national striped catfish production. The test was carried out for two months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila into triploid and diploid striped catfish. The challenge test was carried out by injecting the cultured A. hydrophila with a density of 107 cfu.mL-1 as much as 0.1 mL.ind-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 individuals measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood description were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at a 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila were gram-negative, oxidase and catalase positive, motile, could fermentatively convert sugar, and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid striped catfish are more resistant to infection with A. hydrophila than diploid.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"92 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140421040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.39210
Esther Lourence Brendha Ayal, Kasprijo Kasprijo, R. Fitriadi, D. Ryandini, Mohammad Nurhafid, R. M. Riady, Mira Adyla Anandasari
Amylolytic bacteria play an important role in the ecosystem, especially as probiotic and bioremediation agents in cultivation, as examples can be found in Mina Padi culture. The purpose of this study was to determine the amylolytic bacteria in the waters of the Mina Padi pond. Bacterial isolation began with bacterial sampling, inoculation and isolation of bacteria, calculation of the total abundance of bacteria, observation of bacterial morphology and bacterial purification, and isolation of amylolytic bacteria. The results of the isolation of amylolytic bacteria obtained 3 bacterial isolates capable of producing amylolytic enzymes, namely BA5, BA6, and BA7. The highest index of amylolytic activity was obtained by isolates of BA6 with a medium category of 2.3 cm, and the lowest index was obtained by BA5 with a weak category of 0.3 cm. The average bacterial abundance from each dilution was 2.5 x 103 CFU/mL.
淀粉溶解菌在生态系统中发挥着重要作用，特别是在养殖过程中作为益生菌和生物修复剂，Mina Padi 养殖中就有这样的例子。本研究的目的是确定 Mina Padi 池塘水域中的淀粉溶解菌。细菌分离工作从细菌取样、接种和分离细菌、计算细菌总丰度、观察细菌形态和细菌纯化以及分离淀粉溶解菌开始。淀粉溶解菌的分离结果获得了 3 个能产生淀粉溶解酶的细菌分离株，分别是 BA5、BA6 和 BA7。BA6分离菌的淀粉溶解活性指数最高，为中等2.3厘米；BA5分离菌的淀粉溶解活性指数最低，为弱0.3厘米。每个稀释度的平均细菌数量为 2.5 x 103 CFU/mL。
{"title":"Screening of Amylolytic Bacteria from Mina Padi Aquaculture in Panembangan Village, Cilongok District, Banyumas, Central Java","authors":"Esther Lourence Brendha Ayal, Kasprijo Kasprijo, R. Fitriadi, D. Ryandini, Mohammad Nurhafid, R. M. Riady, Mira Adyla Anandasari","doi":"10.20473/jafh.v13i1.39210","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.39210","url":null,"abstract":"Amylolytic bacteria play an important role in the ecosystem, especially as probiotic and bioremediation agents in cultivation, as examples can be found in Mina Padi culture. The purpose of this study was to determine the amylolytic bacteria in the waters of the Mina Padi pond. Bacterial isolation began with bacterial sampling, inoculation and isolation of bacteria, calculation of the total abundance of bacteria, observation of bacterial morphology and bacterial purification, and isolation of amylolytic bacteria. The results of the isolation of amylolytic bacteria obtained 3 bacterial isolates capable of producing amylolytic enzymes, namely BA5, BA6, and BA7. The highest index of amylolytic activity was obtained by isolates of BA6 with a medium category of 2.3 cm, and the lowest index was obtained by BA5 with a weak category of 0.3 cm. The average bacterial abundance from each dilution was 2.5 x 103 CFU/mL.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"184 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140421076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.49252
Rizal Akbar Hutagalung, Sutrsino Anggoro, Suryanti Suryanti, M. R. Muskananfola
Channa marulioides is one of the species that is facing a population decrease due to high fishing activity. This study aims to determine the value of the length-weight relationship and condition factors of C. marulioides in the Kapuas River as a relevant conservation effort. Several sampling areas in this study were used to represent the Kapuas River, including Kapuas Hulu River in Kapuas Hulu Regency, Putussibau District; The central part of the waters area of Sintang Regency in Sintang Regency; and in the downstream waters of Pontianak City. The study carried out the sampling by analyzing the criteria for specimens with a dimension weight > 500 grams and length > 30 cm, with the same amount for each sex. The results showed that the growth type in the upper and middle waters was positive allometric with a value of b 3.1; however, it is negative allometric in the middle and upstream waters with b values of 2.5 and 1.7. After research, the condition factor of the upstream and middle waters was observed to be in good condition with a value of 1. However, in the downstream waters the condition of the fish was rather bad with a value below 1, namely 0.99. The study concludes there is a decrease in the quality of the aquatic environment and the food availability from the middle waters to the downstream. This is what underlines conservation efforts in the form of domestication activities within the scope of structured fish farming.
{"title":"Length-Weight Relationship and Condition Factor Channa Marulioides (Bleeker, 1851) In Kapuas River – West Kalimantan as a Conservation Aquatic Resources Effort","authors":"Rizal Akbar Hutagalung, Sutrsino Anggoro, Suryanti Suryanti, M. R. Muskananfola","doi":"10.20473/jafh.v13i1.49252","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.49252","url":null,"abstract":"Channa marulioides is one of the species that is facing a population decrease due to high fishing activity. This study aims to determine the value of the length-weight relationship and condition factors of C. marulioides in the Kapuas River as a relevant conservation effort. Several sampling areas in this study were used to represent the Kapuas River, including Kapuas Hulu River in Kapuas Hulu Regency, Putussibau District; The central part of the waters area of Sintang Regency in Sintang Regency; and in the downstream waters of Pontianak City. The study carried out the sampling by analyzing the criteria for specimens with a dimension weight > 500 grams and length > 30 cm, with the same amount for each sex. The results showed that the growth type in the upper and middle waters was positive allometric with a value of b 3.1; however, it is negative allometric in the middle and upstream waters with b values of 2.5 and 1.7. After research, the condition factor of the upstream and middle waters was observed to be in good condition with a value of 1. However, in the downstream waters the condition of the fish was rather bad with a value below 1, namely 0.99. The study concludes there is a decrease in the quality of the aquatic environment and the food availability from the middle waters to the downstream. This is what underlines conservation efforts in the form of domestication activities within the scope of structured fish farming.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"8 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.44281
Tholibah Mujtahidah, Sri Hidayati, Abdul Qadir Jailani, Annisa Novita Sari, Muhammad Tri Aji, Eric Armando
This study's main focus is how the adaptation level affects the treatment of shelter composition in controlled containers. Parameters observed included growth rate, stress level, fish survival rate, and water quality during rearing. The research design used a completely randomized design (CRD) and was analyzed using SPSS. The results showed that the shelter composition was good for the C. limbata survival in experimental group 3 (P3) by placing the addition of sand, stones, gutters and aquatic plants in the aquarium. The effect of the composition of the shelter in a controlled container on the adaptation level of C. limbata was shown in experimental group 3 (P3), which experienced an increase in length of up to 5 mm, and an increase in weight of 25 g and a survival rate of 60%. This value is the best among 1st, 2nd, and control. This shows that the C. limbata can adapt its life to an artificial habitat that is as suitable as its natural habitat.
{"title":"Different Shelters to Domesticate Dwarf Snakehead (Channa limbata, Cuvier 1831) From the Progo River, Magelang, Central Java","authors":"Tholibah Mujtahidah, Sri Hidayati, Abdul Qadir Jailani, Annisa Novita Sari, Muhammad Tri Aji, Eric Armando","doi":"10.20473/jafh.v13i1.44281","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.44281","url":null,"abstract":"This study's main focus is how the adaptation level affects the treatment of shelter composition in controlled containers. Parameters observed included growth rate, stress level, fish survival rate, and water quality during rearing. The research design used a completely randomized design (CRD) and was analyzed using SPSS. The results showed that the shelter composition was good for the C. limbata survival in experimental group 3 (P3) by placing the addition of sand, stones, gutters and aquatic plants in the aquarium. The effect of the composition of the shelter in a controlled container on the adaptation level of C. limbata was shown in experimental group 3 (P3), which experienced an increase in length of up to 5 mm, and an increase in weight of 25 g and a survival rate of 60%. This value is the best among 1st, 2nd, and control. This shows that the C. limbata can adapt its life to an artificial habitat that is as suitable as its natural habitat.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"5 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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