{"title":"Assessment of antibacterial genes of emerging queen Apis mellifera L. induced by the bacterium Paenibacillus larvae larvae","authors":"Sas Gomaa, Ems Barakat, M. Salama, EE El Gohary","doi":"10.17159/2254-8854/2022/a13586","DOIUrl":null,"url":null,"abstract":"This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.","PeriodicalId":7566,"journal":{"name":"African Entomology","volume":" ","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Entomology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17159/2254-8854/2022/a13586","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.
期刊介绍：
African Entomology (ISSN 1021-3589 – print / 2224-8854 – online) replaced the old Journal of the Entomological Society of Southern Africa in 1993. A single volume consisting of two issues (March and September) is published annually. The journal is indexed in all major abstracting journals
African Entomology is a peer reviewed scientific journal that publishes original research articles and short communications on all aspects of entomology, with an emphasis on the advancement of entomology on the African continent.
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