Francisco Javier Flores Gallardo, José Luis Hernández Flores, M. A. Ramos López, Jackeline Lizzeta Arvizu Gómez, V. P. Moreno, José Alberto Rodríguez Morales, Erika Álvarez Hidalgo, Jorge Nuñez Ramírez, George H. Jones, Juan Campos Guillén
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引用次数: 0
Abstract
Summary Illumina MiSeq sequencing of 16S rRNA genes was applied in a molecular diagnostic analysis of healthy and dead larvae obtained from a Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) colony. The results indicated the presence of Enterococcus (Lactobacillales: Enterococcaceae) with an average abundance of 89.2% and 94.8% in healthy and dead larvae, respectively. Pseudomonas (Pseudomonadales: Pseudomonadaceae) and Acetobacter (Rhodospirillales: Acetobacteraceae) were present in lower abundance. Additional metagenomic results revealed the presence in dead larvae of viral sequences related to Alphabaculovirus in high relative abundance compared with healthy larvae. Additionally, viral sequences of tomato leaf curl Laos virus, Heliothis zea (Boddie) (Lepidoptera: Noctuidae) nudivirus, Clostera anachoreta (Denis & Schiffermüller) (Lepidoptera: Notodontidae) granulovirus, and Tipula oleracea L. (Diptera: Tipulidae) nudivirus were detected. Our results show that molecular detection of pathogenic agents is a useful tool during insect rearing as a quality control to avoid the spread or acquisition of potential pathogens. Sumario Nuestros resultados demuestran que a través de un diagnóstico molecular en Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), usando secuenciación del 16S rDNA por Illumina MiSeq, en larvas saludables y muertas mostraron una abundancia relativa alta de Enterococcus (Lactobacillales: Enterococcaceae) con un promedio de 89.2% and 94.8% respectivamente. Mientras que Pseudomonas (Pseudomonadales: Pseudomonadaceae) y Acetobacter (Rhodospirillales: Acetobacteraceae) presentaron menor abundancia relativa. Adicionalmente, resultados de metagenomica mostraron en larvas muertas secuencias de virus relacionados con Alphabaculovirus con una abundancia relativa muy alta comparada con las larvas saludables. Se detectaron secuencias virales relacionadas al Encrespamiento amarillo de la hoja del tomate, Heliothis zea (Boddie) (Lepidoptera: Noctuidae) nudivirus, Clostera anachoreta (Denis & Schiffermüller) (Lepidoptera: Notodontidae) granulovirus and Tipula oleracea L. (Diptera: Tipulidae) nudivirus. Nuestros resultados demuestran que una detección molecular de agentes patogénicos es primordial durante la crianza de insectos como un control de calidad para evitar su dispersión o adquisición.
期刊介绍：
Florida Entomologist is the official journal of the Florida Entomological Society. Volumes 1-3 were published under the name The Florida Buggist. The Florida Entomological Society still produces the traditionally printed version of Florida Entomologist, but you can also view, search, or print any article published since June 1917 by accessing online files. Web access is made possible by the Society’s electronic publication project begun in 1993
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