{"title":"Carbon Nanotubes/Gold Nanoclusters Based “Turn on” FRET Fluorescent Assay for miRNA Detection","authors":"Lili Chen, Lingling Chen, Qiuhua Yang, Fengrong Zhang, Q. Qu","doi":"10.1166/sam.2023.4482","DOIUrl":null,"url":null,"abstract":"This paper presents a “turn on” fluorescent assay with high sensitivity and rapidity for testing miRNA-195 on the basis of a novel FRET pair consisting of Lys-Au NCs (which have been successfully obtained through less than 2.5 min of synthesis via microwave (MW)-assisted\n technology) and carbon nanotubes (CNTs). Based on this FRET pair, given a detection limit set at 0.36 nM, miRNA-195 can be quantified in the range of 1–10000 nM. Moreover, the level of miRNA-195 in human serum was measured by virtue of this method, and a recovery of 98.6%–102.4%\n was achieved, demonstrating that the method put forward in this paper was applicable to actual samples for quantitatively examining miRNA-195.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science of Advanced Materials","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1166/sam.2023.4482","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
This paper presents a “turn on” fluorescent assay with high sensitivity and rapidity for testing miRNA-195 on the basis of a novel FRET pair consisting of Lys-Au NCs (which have been successfully obtained through less than 2.5 min of synthesis via microwave (MW)-assisted
technology) and carbon nanotubes (CNTs). Based on this FRET pair, given a detection limit set at 0.36 nM, miRNA-195 can be quantified in the range of 1–10000 nM. Moreover, the level of miRNA-195 in human serum was measured by virtue of this method, and a recovery of 98.6%–102.4%
was achieved, demonstrating that the method put forward in this paper was applicable to actual samples for quantitatively examining miRNA-195.
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