The upregulation of peripheral CD3-CD56+CD16+ natural killer cells correlates with Th1/Th2 imbalance in asthma patients during acute upper respiratory viral infections.

IF 2.9 4区 医学 Q3 IMMUNOLOGY BMC Immunology Pub Date : 2023-10-21 DOI:10.1186/s12865-023-00575-y
Meixuan Liu, Yunxuan Zhang, Yunqian Hu, Zhongliang Guo, Lin Dong
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Abstract

Purpose: The aim of this study is to clarify the changes of peripheral CD3-CD56+CD16+ NK cells and their correlation with Th1/Th2 immunity profiles in asthma during the phase of acute upper respiratory viral infections (AURVIs).

Methods: Peripheral venous blood and induced sputum samples were collected from 56 mild asthma patients, 49 asthma patients with AURVIs and 50 healthy subjects. Peripheral CD3-CD56+CD16+ NK cells were monitored by flow cytometry during the course of acute viral infections. Meanwhile, the induced sputum Th2 cytokines IL-4 and IL-5, and Th1 cytokine IFN-γ were also detected by ELISA assay.

Results: The asthmatics had lower levels of peripheral CD3-CD56+CD16+ NK cells populations as well as higher induced sputum cytokines (IL-4, IL-5 and IFN-γ) compared to healthy controls at baseline. Upon upper respiratory viral infections, peripheral CD3-CD56+CD16+ NK cells numbers in asthma patients sharply elevated on day 3 and slowly decreased by day 14, in accordance with induced sputum IFN-γ changes. IL-4 and IL-5 levels spiked much later (day 8) and lasted until day 14. Compared with asthma alone group, the IFN-γ/IL-4 and IFN-γ/IL-5 ratios of the asthma patients with AURVIs on day 1 were higher and peaked on day 3. The changes of peripheral CD3-CD56+CD16+ NK cells proportions positively correlated with the IFN-γ/IL-4 and IFN-γ/IL-5 ratios on day 1 to day 3 in asthma subsequent to upper respiratory viral infections.

Conclusions: Our findings showed an imbalanced Th1/Th2 immunity in airways of asthma with acute upper respiratory viral infections. Upregulated peripheral CD3-CD56+CD16+ NK cells play a crucial role in biased Th1 immunity of airways in asthma during the acute phase of viral infections. The anti-viral Th1 immunity by targeting NK cells may be a possible therapeutic option for virus-induced asthma exacerbation.

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急性上呼吸道病毒感染期间哮喘患者外周CD3-CD56+CD16+自然杀伤细胞的上调与Th1/Th2失衡相关。
目的:本研究旨在阐明急性上呼吸道病毒感染(AURVIs)期间哮喘患者外周CD3-CD56+CD16+NK细胞的变化及其与Th1/Th2免疫谱的相关性。在急性病毒感染过程中通过流式细胞术监测外周CD3-CD56+CD16+NK细胞。同时,ELISA法检测诱导痰中Th2细胞因子IL-4和IL-5,以及Th1细胞因子IFN-γ。结果:与基线健康对照组相比,哮喘患者外周CD3-CD56+CD16+NK细胞群水平较低,诱导的痰液细胞因子(IL-4、IL-5和IFN-γ)水平较高。在上呼吸道病毒感染后,哮喘患者的外周CD3-CD56+CD16+NK细胞数量在第3天急剧增加,在第14天缓慢减少,这与诱导的痰IFN-γ变化一致。IL-4和IL-5水平在很久之后(第8天)飙升并持续到第14天。与单纯哮喘组相比,AURVIs哮喘患者在第1天的IFN-γ/IL-4和IFN-γ-IL-5比值较高,并在第3天达到峰值。外周CD3-CD56+CD16+NK细胞比例的变化与上呼吸道病毒感染后哮喘第1天至第3天的IFN-γ/IL-4和IFN-γ/IL-5比例呈正相关。结论:我们的研究结果显示,急性上呼吸道病毒感染的哮喘气道中Th1/Th2免疫失衡。在病毒感染的急性期,上调的外周CD3-CD56+CD16+NK细胞在哮喘气道的偏向性Th1免疫中起着至关重要的作用。通过靶向NK细胞的抗病毒Th1免疫可能是病毒诱导的哮喘恶化的一种可能的治疗选择。
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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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