{"title":"Down-regulation of the <i>FTO</i> gene in follicular fluid of infertile women with ovarian endometriosis.","authors":"Yanmei Li, Naihui Wang, Yuanxue Jing, Jiajing He, Fangfang Li, Xuehong Zhang","doi":"10.1080/09513590.2023.2269273","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate FTO concentrations in follicular fluid (FF) of women with ovarian endometriosis (OE) and controls women without OE undergoing <i>in vitro</i> fertilization-embryo transfer (IVF-ET).</p><p><strong>Methods: </strong>FTO concentrations in FF were measured in 74 patients (37 in the control group and 37 in the OE group) by ELISA. We measured the expression of FTO in GCs of 40 patients (19 in the control group and 21 in the OE group) by RT-qPCR. The level of m<sup>6</sup>A in GCs was measured in 20 patients (10 in the control group and 10 in the OE group) by colorimetry.</p><p><strong>Results: </strong>Compared with the control group, FTO concentrations in FF (6.92 ± 0.44 <i>vs.</i> 5.67 ± 0.40 ng/ml) (<i>p</i> <.05) and FTO mRNA level in GCs of OE group were decreased significantly (<i>p</i> <.05), and the level of m<sup>6</sup>A was increased (0.21 ± 0.01 <i>vs.</i> 0.17 ± 0.03 ng) (<i>p</i> >.05).</p><p><strong>Conclusions: </strong>The FTO concentrations in FF of infertility women with OE are decreased, which may be related to the impaired oocyte quality in endometriosis patients.</p>","PeriodicalId":12865,"journal":{"name":"Gynecological Endocrinology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gynecological Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09513590.2023.2269273","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/10/23 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To evaluate FTO concentrations in follicular fluid (FF) of women with ovarian endometriosis (OE) and controls women without OE undergoing in vitro fertilization-embryo transfer (IVF-ET).
Methods: FTO concentrations in FF were measured in 74 patients (37 in the control group and 37 in the OE group) by ELISA. We measured the expression of FTO in GCs of 40 patients (19 in the control group and 21 in the OE group) by RT-qPCR. The level of m6A in GCs was measured in 20 patients (10 in the control group and 10 in the OE group) by colorimetry.
Results: Compared with the control group, FTO concentrations in FF (6.92 ± 0.44 vs. 5.67 ± 0.40 ng/ml) (p <.05) and FTO mRNA level in GCs of OE group were decreased significantly (p <.05), and the level of m6A was increased (0.21 ± 0.01 vs. 0.17 ± 0.03 ng) (p >.05).
Conclusions: The FTO concentrations in FF of infertility women with OE are decreased, which may be related to the impaired oocyte quality in endometriosis patients.
期刊介绍：
Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction
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