{"title":"Walking leg regeneration in the sea spider Nymphon brevirostre Hodge, 1863 (Pycnogonida)","authors":"Maria Petrova, Ekaterina Bogomolova","doi":"10.1016/j.asd.2023.101310","DOIUrl":null,"url":null,"abstract":"<div><p>Regeneration is widespread across all animal taxa, but patterns of its distribution and key factors determining regeneration capabilities stay enigmatic. A comparative approach could shed light on the problem, but its efficacy is limited by the fact that data is only available on a few species from derived taxa. Pycnogonida are nested basally within the Chelicerata. They can shed and replace their walking legs and have a high regeneration capacity. In this work, we carried careful observation on leg appendotomy and regeneration processes in a sea spider under laboratory settings. The limb structure and <em>in vivo</em> observation reveal autotomy as the most likely appendotomy mechanism. High regeneration capabilities were ascertained: an anatomically normal but small leg appeared in a single molting cycle and the full functionality regained in 2–3 cycles. Wound closure after appendotomy in <em>N. brevirostre</em> primarily relies on hemolymph coagulation, which apparently differs from both xiphosurans and crustaceans. Regeneration is provided by proliferation in the leg cutpiece. Regenerative morphogenesis resembles the normal ontogenetic morphogenesis of a walking leg, but accelerated. Unlike in most arthropods, in <em>N. brevirostre</em>, regeneration does not necessarily correspond to the molting cycle, inferring a plesiomorphic state.</p></div>","PeriodicalId":55461,"journal":{"name":"Arthropod Structure & Development","volume":"77 ","pages":"Article 101310"},"PeriodicalIF":1.7000,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arthropod Structure & Development","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1467803923000774","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Regeneration is widespread across all animal taxa, but patterns of its distribution and key factors determining regeneration capabilities stay enigmatic. A comparative approach could shed light on the problem, but its efficacy is limited by the fact that data is only available on a few species from derived taxa. Pycnogonida are nested basally within the Chelicerata. They can shed and replace their walking legs and have a high regeneration capacity. In this work, we carried careful observation on leg appendotomy and regeneration processes in a sea spider under laboratory settings. The limb structure and in vivo observation reveal autotomy as the most likely appendotomy mechanism. High regeneration capabilities were ascertained: an anatomically normal but small leg appeared in a single molting cycle and the full functionality regained in 2–3 cycles. Wound closure after appendotomy in N. brevirostre primarily relies on hemolymph coagulation, which apparently differs from both xiphosurans and crustaceans. Regeneration is provided by proliferation in the leg cutpiece. Regenerative morphogenesis resembles the normal ontogenetic morphogenesis of a walking leg, but accelerated. Unlike in most arthropods, in N. brevirostre, regeneration does not necessarily correspond to the molting cycle, inferring a plesiomorphic state.
期刊介绍:
Arthropod Structure & Development is a Journal of Arthropod Structural Biology, Development, and Functional Morphology; it considers manuscripts that deal with micro- and neuroanatomy, development, biomechanics, organogenesis in particular under comparative and evolutionary aspects but not merely taxonomic papers. The aim of the journal is to publish papers in the areas of functional and comparative anatomy and development, with an emphasis on the role of cellular organization in organ function. The journal will also publish papers on organogenisis, embryonic and postembryonic development, and organ or tissue regeneration and repair. Manuscripts dealing with comparative and evolutionary aspects of microanatomy and development are encouraged.