Ratiometric H2S probe that can be recognized by the naked eye and its application in cell imaging

IF 1.4 4区 化学 Q4 CHEMISTRY, INORGANIC & NUCLEAR Phosphorus, Sulfur, and Silicon and the Related Elements Pub Date : 2023-01-01 DOI:10.1080/10426507.2023.2199995
Fangfang Luo , Le Wang , Xiao Zhang , Ping Xu , Xiao Wang , Yi Qu
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Abstract

A ratiometric dual-mode probe NI-SH based on 1,8-Naphthalimide was prepared for H2S detection. When treated with H2S, the fluorescence of NI-SH changes from blue (456 nm) to green (540 nm), and displays significant color variation (colorless to yellow) that can visible to the naked eye. Further investigation shows that the NI-SH has high sensitivity, selectivity, and low detection limit at about 0.2 μM, and with large Stokes shift (84 nm). The intermolecular charge transfer (ICT) response mechanism of NI-SH was further studied by analyzing the distributions of the molecular orbital. Besides, NI-SH exhibits a ratiometric fluorescence response in living Hela cells.

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肉眼可识别的比率H2S探针及其在细胞成像中的应用
制备了一种基于1,8-萘酰亚胺的比率双模式探针NI-SH用于H2S检测。当用H2S处理时,NI-SH的荧光由蓝色(456 nm)变为绿色(540 nm)并且显示肉眼可见的显著的颜色变化(无色到黄色)。进一步的研究表明,NI-SH具有高灵敏度、高选择性和低检测限,约为0.2 μM,并且具有大的斯托克斯位移(84 nm)。通过分析分子轨道的分布,进一步研究了NI-SH的分子间电荷转移(ICT)响应机制。此外,NI-SH在活的Hela细胞中表现出比率荧光反应。
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来源期刊
CiteScore
2.60
自引率
7.70%
发文量
103
审稿时长
2.1 months
期刊介绍: Phosphorus, Sulfur, and Silicon and the Related Elements is a monthly publication intended to disseminate current trends and novel methods to those working in the broad and interdisciplinary field of heteroatom chemistry.
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