Real time microcalorimetric profiling of prebiotic inulin metabolism

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-06-10 DOI:10.1016/j.fhfh.2023.100141
Mansa Fredua-Agyeman , Simon Gaisford
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Abstract

The in vitro assessment of prebiotics involves elaborate microbiological techniques or a combination of culture techniques and molecular methods. In this study, the isothermal microcalorimeter, an instrument which can monitor the real time growth of bacteria was applied to investigate the prebiotic effect of inulin in real time. Fresh and standardized frozen faecal slurries were prepared, placed and monitored in the isothermal microcalorimeter. The faecal samples and commercial probiotic strains Lactobacillus acidophilus LA-5®, Bifidobacterium lactis BB-12® were cultured in a mixed medium of cooked meat medium (CMM) and brain heart infusion (BHI) broth with and without supplementation with inulin and monitored in the microcalorimeter. The results showed power-time (p-t) curves that were characteristic for the samples. The p-t curves of the fresh and frozen faecal samples were similar. Augmented microbial activity was observed when the faecal sample was inoculated into CMM-BHI mixed broth with significant enhancement of microbial activity detected in the presence of inulin which was reproducible. Deconvoluted p-t curves showed multiple peaks with time and intensity variance depending on presence or absence of inulin suggesting possible differences in utilization of inulin by the different groups of bacteria in the polymicrobial sample. P-t curves of the pure species did not show any significant change when inulin was supplemented into the medium likely due to the inability of the bacteria to primarily utilize inulin.

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益生元菊糖代谢的实时微热量分析
益生元的体外评估涉及精细的微生物技术或培养技术与分子方法的结合。本研究采用可实时监测细菌生长的等温微量热量计,实时研究菊粉的益生元作用。制备新鲜和标准化的冷冻粪便浆,放置在等温微量热量计中并进行监测。将粪便样品和商业益生菌菌株嗜酸乳杆菌LA-5®、乳酸双歧杆菌BB-12®在添加和不添加菊粉的熟肉培养基(CMM)和脑心浸液(BHI)肉汤的混合培养基中培养,并在微量热量计中监测。结果显示了功率-时间(p-t)曲线,这是样品的特征。新鲜和冷冻粪便样品的p-t曲线相似。当将粪便样品接种到CMM-BHI混合肉汤中时,观察到微生物活性增强,在菊粉存在下检测到的微生物活性显著增强,这是可重复的。去卷积的p-t曲线显示出多个峰值,其随时间和强度的变化取决于菊粉的存在与否,这表明多微生物样品中不同细菌组对菊粉的利用可能存在差异。当菊粉被补充到培养基中时,纯物种的P-t曲线没有显示出任何显著的变化,这可能是由于细菌不能主要利用菊粉。
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CiteScore
4.50
自引率
0.00%
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0
审稿时长
61 days
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