Enhancement mechanism of biocathodes on nitrification in the bioelectrochemical system from a microbial perspective

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Environmental Technology & Innovation Pub Date : 2023-08-11 DOI:10.1016/j.eti.2023.103330

Shan Huang , Jingran Zhang , Huimin Zhang , Chuqiao Wang , Chenglong Zou , Dingchang Li

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Abstract

Biocathodes in bioelectrochemical systems (BESs) have been reported to enhance nitrogen (N) removal. However, the complex multi-parameter variables in BESs have increased the difficulty of studying the enhanced mechanism of nitrification using biocathodes. This study aimed to construct a range of potentiostatic biocathodes (−400, −200, ＋200, and ＋400 mV vs Ag/AgCl) with a dissolved O₂ concentration of 2–3 mg L⁻¹ using a potentiostat to further clarify the mechanism. The mechanism of how biocathodes influence microorganisms was elucidated from multiple perspectives, including variations in N concentration, electrochemistry, high-throughput sequencing, and PICRUSt2 prediction. The biocathodes were found to increase the nitrification rate by improving the abundance of nitrification-related bacteria (unidentified_Nitrospiraceae, Thauera, Nitrosomonas, Prosthecobacter, and Stenotrophomonas), the expression of genes related to ammonia oxidation and nitrite oxidation (pmoABC, hao, nirk, ncd2, and npd), and the activity of ammonia oxidase (AMO) and nitrite oxidase (NOR) in the biocathodes. The results of this study might provide crucial academic support and demonstration in facilitating the application of BESs and realizing the deep treatment of wastewater.

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从微生物角度看生物阴极对生物电化学系统硝化作用的增强机理

据报道，生物电化学系统（BES）中的生物阴极可以增强氮（N）的去除。然而，BESs中复杂的多参数变量增加了利用生物阴极研究硝化作用增强机制的难度。本研究旨在使用恒电位仪构建一系列溶解O2浓度为2–3 mg L−1的恒电位生物阴极（−400、−200、＋200和＋400 mV vs Ag/AgCl），以进一步阐明其机制。从多个角度阐明了生物病原体如何影响微生物的机制，包括氮浓度的变化、电化学、高通量测序和PICRUSt2预测。发现生物病原体通过提高硝化相关细菌（未识别的氮螺旋菌科、Thauera、硝化单胞菌、Prosthecobacter和狭窄单胞菌）的丰度、与氨氧化和亚硝酸盐氧化相关的基因（pmoABC、hao、nirk、ncd2和npd）的表达来提高硝化速率，以及氨氧化酶（AMO）和亚硝酸盐氧化酶（NOR）在生物阴极中的活性。该研究结果可能为BESs的应用和污水深度处理的实现提供重要的学术支持和示范。

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来源期刊

Environmental Technology & Innovation Environmental Science-General Environmental Science

CiteScore

14.00

自引率

4.20%

发文量

435

审稿时长

74 days

期刊介绍： Environmental Technology & Innovation adopts a challenge-oriented approach to solutions by integrating natural sciences to promote a sustainable future. The journal aims to foster the creation and development of innovative products, technologies, and ideas that enhance the environment, with impacts across soil, air, water, and food in rural and urban areas. As a platform for disseminating scientific evidence for environmental protection and sustainable development, the journal emphasizes fundamental science, methodologies, tools, techniques, and policy considerations. It emphasizes the importance of science and technology in environmental benefits, including smarter, cleaner technologies for environmental protection, more efficient resource processing methods, and the evidence supporting their effectiveness.