UHPLC-Q-TOF analysis of pyrrolizidine alkaloids in North-Macedonian honey.

IF 2.5 3区农林科学 Q2 CHEMISTRY, APPLIED Food additives & contaminants. Part B, Surveillance Pub Date : 2024-03-01 Epub Date: 2024-02-12 DOI:10.1080/19393210.2023.2266701

Marinela Cvetanoska, Marijana Pocrnić, Marina Stefova, Nives Galić, Jasmina Petreska Stanoeva

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Abstract

Honey contaminated with pyrrolizidine alkaloids (PAs) could pose a risk for human consumption, being a widely consumed food product. A fast and simple LC/MS method for the analysis of pyrrolizidine alkaloids in honey was optimised to collect occurrence data. The extraction efficiency was evaluated by a systematic study of multiple solvent mixtures and clean-up procedures. The best results for PA extraction were obtained using a formic acid/methanol mixture with subsequent clean-up by the QuEChERS method, resulting in a mean recovery range of 91.8-102%. The method validation showed satisfactory intra-day (RSD < 5.1%) and inter-day precision (RSD < 9.1%). The proposed method was applied to 14 samples. A total of six PAs and two N-oxides were detected, with levels between 89 and 8188 µg/kg. This assessment highlights the potential risk of intoxication and the need for further investigations regarding an effective quality system for manufacturers to control PAs in honey.

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北马其顿蜂蜜中吡咯烷啶生物碱的UHPLC-Q-TOF分析。

被吡咯烷啶生物碱（PA）污染的蜂蜜作为一种广泛消费的食品，可能对人类消费构成风险。为了收集蜂蜜中吡咯烷啶生物碱的发生数据，优化了一种快速简便的LC/MS分析方法。通过对多种溶剂混合物和清洁程序的系统研究来评估提取效率。PA提取的最佳结果是使用甲酸/甲醇混合物，随后通过QuEChERS方法进行净化，平均回收率为91.8-102%。方法验证显示日内（RSD 检测到氮氧化物，含量在89至8188之间 µg/kg。该评估强调了潜在的中毒风险，并需要进一步调查制造商控制蜂蜜中PAs的有效质量体系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food additives & contaminants. Part B, Surveillance 农林科学-毒理学

CiteScore

5.30

自引率

10.30%

发文量

审稿时长

2.7 months

期刊介绍： Food Additives & Contaminants: Part B publishes surveillance data indicating the presence and levels of occurrence of designated food additives, residues and contaminants in foods, food supplements and animal feed. Data using validated methods must meet stipulated quality standards to be acceptable and must be presented in a prescribed format for subsequent data-handling. Food Additives & Contaminants: Part B restricts its scope to include certain classes of food additives, residues and contaminants. This is based on a goal of covering those areas where there is a need to record surveillance data for the purposes of exposure and risk assessment. The scope is initially restricted to: Additives - food colours, artificial sweeteners, and preservatives; Residues – veterinary drug and pesticide residues; Contaminants – metals, mycotoxins, phycotoxins, plant toxins, nitrate/nitrite, PCDDs/PCFDs, PCBs, PAHs, acrylamide, 3-MPCD and contaminants derived from food packaging. Readership: The readership includes scientists involved in all aspects of food safety and quality and particularly those involved in monitoring human exposure to chemicals from the diet. Papers reporting surveillance data in areas other than the above should be submitted to Part A . The scope of Part B will be expanded from time-to-time to ensure inclusion of new areas of concern.