NaCl-induced modulation of species distribution in a mixed P. aeruginosa / S. aureus /B. cepacia biofilm

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2023-09-04 DOI:10.1016/j.bioflm.2023.100153
Jeanne Trognon , Maya Rima , Barbora Lajoie , Christine Roques , Fatima El Garah
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Abstract

Pseudomonas aeruginosa, Staphylococcus aureus, and Burkholderia cepacia are notorious pathogens known for their ability to form resilient biofilms, particularly within the lung environment of cystic fibrosis (CF) patients. The heightened concentration of NaCl, prevalent in the airway liquid of CF patients' lungs, has been identified as a factor that promotes the growth of osmotolerant bacteria like S. aureus and dampens host antibacterial defenses, thereby fostering favorable conditions for infections.

In this study, we aimed to investigate how increased NaCl concentrations impact the development of multi-species biofilms in vitro, using both laboratory strains and clinical isolates of P. aeruginosa, S. aureus, and B. cepacia co-cultures. Employing a low-nutrient culture medium that fosters biofilm growth of the selected species, we quantified biofilm formation through a combination of adherent CFU counts, qPCR analysis, and confocal microscopy observations.

Our findings reaffirmed the challenges faced by S. aureus in establishing growth within 1:1 mixed biofilms with P. aeruginosa when cultivated in a minimal medium. Intriguingly, at an elevated NaCl concentration of 145 mM, a symbiotic relationship emerged between S. aureus and P. aeruginosa, enabling their co-existence. Notably, this hyperosmotic environment also exerted an influence on the interplay of these two bacteria with B. cepacia. We demonstrated that elevated NaCl concentrations play a pivotal role in orchestrating the distribution of these three species within the biofilm matrix.

Furthermore, our study unveiled the beneficial impact of NaCl on the biofilm growth of clinically relevant mucoid P. aeruginosa strains, as well as two strains of methicillin-sensitive and methicillin-resistant S. aureus. This underscores the crucial role of the microenvironment during the colonization and infection processes. The results suggest that hyperosmotic conditions could hold the key to unlocking a deeper understanding of the genesis and behavior of CF multi-species biofilms.

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NaCl诱导铜绿假单胞菌/金黄色葡萄球菌/B混合菌中物种分布的调节。洋葱生物膜
铜绿假单胞菌、金黄色葡萄球菌和洋葱伯克霍尔德菌是臭名昭著的病原体,以其形成弹性生物膜的能力而闻名,尤其是在囊性纤维化(CF)患者的肺部环境中。CF患者肺部气道液体中普遍存在的NaCl浓度升高,已被确定为促进金黄色葡萄球菌等耐渗细菌生长并抑制宿主抗菌防御的因素,从而为感染创造有利条件。在这项研究中,我们旨在研究NaCl浓度的增加如何影响体外多物种生物膜的形成,使用铜绿假单胞菌、金黄色葡萄球菌和洋葱芽孢杆菌的实验室菌株和临床分离株共同培养。使用促进所选物种生物膜生长的低营养培养基,我们通过结合粘附CFU计数、qPCR分析和共聚焦显微镜观察来量化生物膜的形成。我们的研究结果重申了金黄色葡萄球菌在最小培养基中培养时,在与铜绿假单胞菌1:1混合的生物膜中生长所面临的挑战。有趣的是,在145mM的NaCl浓度升高时,金黄色葡萄球菌和铜绿假单胞菌之间出现了共生关系,使它们能够共存。值得注意的是,这种高渗环境也对这两种细菌与洋葱芽孢杆菌的相互作用产生了影响。我们证明,升高的NaCl浓度在协调这三种物种在生物膜基质中的分布方面发挥着关键作用。此外,我们的研究揭示了NaCl对临床相关的粘液类铜绿假单胞菌菌株以及两株对甲氧西林敏感和耐甲氧西林的金黄色葡萄球菌的生物膜生长的有益影响。这突出了微环境在定植和感染过程中的关键作用。结果表明,高渗条件可能是解开对CF多物种生物膜的起源和行为的更深入理解的关键。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
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