Evaluation of soybean peroxidase - Copper phosphate mediated organic-inorganic hybrid for hydrogen peroxide biosensor application

Sensors International Pub Date : 2023-01-01 DOI:10.1016/j.sintl.2023.100242

Sunil Bhapkar , Upasana Choudhari , Umesh Jadhav , Shweta Jagtap

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引用次数: 1

Abstract

An electrochemical biosensor for H₂O₂ detection was developed by using soybean peroxidase-copper phosphate mediated organic inorganic hybrid (OIH). The characterization of OIH was carried out by FESEM and FTIR. FESEM analysis showed a flower-like porous morphology of the OIH and FTIR confirmed the presence of soybean peroxidase and copper phosphate in the OIH. For sensor development, the paste of OIH was formulated in the presence of phosphate buffered saline (PBS) and was screen printed on indium tin oxide (ITO) coated glass substrate. Cyclic voltammetry analysis showed that the developed biosensor could detect H₂O₂ in the linear range of 20–100 μM with R² value of 0.963. The limit of detection (LOD) and sensitivity values calculated for H₂O₂ are 0.19 μM and 27.44 μA/(μM.cm2) respectively. Along with cyclic voltammetry experiments, electrochemical impedance spectroscopy (EIS) analysis was also carried out to study the sensing mechanism. The developed biosensor showed better selectivity towards H₂O₂ when tested against d-glucose, l-tyrosine, l-lysine, and l-ascorbic acid.

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大豆过氧化物酶-磷酸铜介导的有机-无机杂化物在过氧化氢生物传感器中的应用评价

利用大豆过氧化物酶-磷酸铜介导的有机-无机杂化物（OIH），研制了一种检测H2O2的电化学生物传感器。利用FESEM和FTIR对OIH进行了表征。FESEM分析显示OIH呈花状多孔形态，FTIR证实OIH中存在大豆过氧化物酶和磷酸铜。对于传感器开发，在磷酸盐缓冲盐水（PBS）的存在下配制OIH的糊状物，并将其丝网印刷在铟锡氧化物（ITO）涂覆的玻璃基板上。循环伏安法分析表明，所开发的生物传感器可以在20–100的线性范围内检测H2O2μM，R2值为0.963。H2O2的检测限（LOD）和灵敏度计算值为0.19μM和27.44。除了循环伏安法实验外，还进行了电化学阻抗谱（EIS）分析，以研究其传感机理。当对d-葡萄糖、l-酪氨酸、l-赖氨酸和l-抗坏血酸进行测试时，所开发的生物传感器显示出对H2O2更好的选择性。

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Sensors International

CiteScore

17.40

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