Exploring the possibility of a proprietary black cumin oil extract as a Dual Orexin Receptor Antagonist in restoring stress-sleep balance on stress-induced sleep deprived animals

IF 2.4 Q3 NUTRITION & DIETETICS PharmaNutrition Pub Date : 2023-09-13 DOI:10.1016/j.phanu.2023.100357

Saneha OR , Priya Krishna , Syam Das S , Krishnakumar IM , Litty Joseph

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Abstract

Background

Orexins act as a molecular switch for the release of cortisol/corticosterone in response to a stress stimulus and hence to regulate sleep/wake cycle. Orexin agonism during the day promotes wakefulness, and Dual Orexin Receptor Antagonists (DORA) can promote sleep signals by enhancing melatonin, which is an inhibitor of orexin. It was reported that a proprietary black cumin (Nigella sativa) oil (BCO-5) alleviated stress and improved sleep quality. The present study investigated the mechanism of action of BCO-5 using stress-induced and sleep-deprived model of rats.

Methods

Adult Sprague Dawley rats (n = 24) were randomised into 4 groups (Group I – Sham; Group II – Stress-induced group; Group III – BCO-5 treated normal animals; Group IV – Stress + BCO-5 (20 mg/kg b. wt.) for 14 days and monitored the behaviour and biochemical markers.

Results

The co-supplementation of BCO-5 significantly decreased the body weight, locomotor activity, rearing and grooming frequencies among Group IV animals significantly compared to Group II. The observed behaviour was also correlated with the significant decrease in orexin, corticosterone and c-fos expression levels, while an increase was observed in melatonin concentration.

Conclusion

Our results support the plausible role of BCO-5 as a DORA to manage stress and improve sleep.

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探索一种专有的黑孜然油提取物作为双Orexin受体拮抗剂在恢复压力诱导的睡眠剥夺动物的压力睡眠平衡中的可能性

背景Orexins作为一种分子开关，在压力刺激下释放皮质醇/皮质酮，从而调节睡眠/觉醒周期。白天的食欲素激动剂可以促进清醒，双食欲素受体拮抗剂（DORA）可以通过增强褪黑素来促进睡眠信号，褪黑素是食欲素的抑制剂。据报道，一种专有的黑孜然（Nigella sativa）油（BCO-5）缓解了压力，改善了睡眠质量。本研究采用应激诱导和睡眠剥夺大鼠模型，探讨BCO-5的作用机制。方法将成年Sprague-Dawley大鼠（n=24）随机分为4组（I组–Sham；II组–应激诱导组；III组–BCO-5处理的正常动物；IV组–应激+BCO-5（20mg/kg b.wt.），为期14天，并监测其行为和生化标志物。结果与第二组相比，共同补充BCO-5显著降低了第四组动物的体重、运动活性、饲养和梳理频率。观察到的行为还与食欲素、皮质酮和c-fos表达水平的显著降低有关，而褪黑素浓度的增加则观察到。结论我们的研究结果支持BCO-5作为DORA在管理压力和改善睡眠方面的合理作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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