In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation

Abstract

Fast-growing forest species with multiple uses, like bamboo, have aroused interest for their silvicultural applications. Bamboo species are a valuable source of renewable raw material, and Bambusa vulgaris is an economically important species. However, there are limitations to large-scale cloning of adult-selected genotypes. This study aimed to evaluate the in vitro cloning of Bambusa vulgaris in different culture systems, sucrose and activated charcoal supplementation by the inter-simple-sequence repeat (ISSR) molecular markers. In vitro bud multiplication and shoot elongation were evaluated in three cultivation systems: semi-solid and liquid culture media, and temporary immersion bioreactor (TIB). The sucrose concentrations, 0 and 30 g L⁻¹ were evaluated in the stages. Both the culture media were supplemented with 2.0 mg L⁻¹ benzylaminopurine (BAP) and 0.5 mg L⁻¹ α-naphthalene acetic acid (NAA). The absence and presence of activated charcoal (100 mg L⁻¹) were evaluated in the in vitro rooting. MS culture medium was supplemented with 2 mg L⁻¹ indole-3-butyric acid (IBA), 1.0 mg L⁻¹ NAA, and 0.5 mg L⁻¹ BAP. Semi-solid culture medium supplemented with 30 g L⁻¹ of sucrose presented superior emission of bud per explant. Liquid culture medium supplemented with 30 g L⁻¹ of sucrose presented the most elongated shoots. Activated charcoal in the culture medium did not influence the adventitious rooting. Micropropagated plants showed genetic fidelity and were clones of the adult selected plant.