Phylogenetic structure and molecular species delimitation hint a complex evolutionary history in an Alpine endemic Niphargus clade (Crustacea, Amphipoda)

IF 1.2 3区 生物学 Q2 ZOOLOGY Zoologischer Anzeiger Pub Date : 2023-09-01 DOI:10.1016/j.jcz.2023.07.001
Mara Knüsel , Špela Borko , Roman Alther , Alice Salussolia , Jean-François Flot , Florian Altermatt , Cene Fišer , Fabio Stoch
{"title":"Phylogenetic structure and molecular species delimitation hint a complex evolutionary history in an Alpine endemic Niphargus clade (Crustacea, Amphipoda)","authors":"Mara Knüsel ,&nbsp;Špela Borko ,&nbsp;Roman Alther ,&nbsp;Alice Salussolia ,&nbsp;Jean-François Flot ,&nbsp;Florian Altermatt ,&nbsp;Cene Fišer ,&nbsp;Fabio Stoch","doi":"10.1016/j.jcz.2023.07.001","DOIUrl":null,"url":null,"abstract":"<div><p>Subterranean fauna is an important contributor to the global fauna, but it is still understudied and a large part of its taxonomy is not yet resolved. One species complex with unresolved taxonomy is the groundwater amphipod <em>Niphargus ruffoi</em>, endemic to the Alpine chain. Here, we used new samples from across the Alpine arc to review the taxonomic status of the entire clade, including the species <em>N. ruffoi</em> and <em>Niphargus arolaensis</em>. We sequenced four genetic markers from the collected specimens, assessed the phylogenetic position of <em>N. ruffoi</em> within the genus, and studied the structure of this species complex using four molecular species delimitation methods. We tested for recombination using the alignments of the concatenated nuclear rDNA genes. The phylogenetic analyses revealed high support for the monophyly of the studied species complex, defining two lineages (i.e., <em>N. arolaensis</em> and <em>N. ruffoi</em>) within the clade. Molecular species delimitation methods suggested that <em>N. arolaensis</em> is a single species, while <em>N. ruffoi</em> should be considered as a species complex of three (using ITS) to eight (using COI) putative species. Moreover, we found a discrepancy between the different nuclear ribosomal DNA markers, indicating a possible recombination with fragments of 28S DNA of <em>N. ruffoi</em> s. lat. present in the genome of <em>N. arolaensis</em>. For the above-mentioned reasons, the internal phylogenetic structure of <em>N. ruffoi</em> s. lat. could not be fully resolved. Moreover, no clear morphological evidence supported the molecular species delimitation. Consequently, no taxonomic changes were proposed. We postulate that this complex scenario was influenced by Pleistocene climate oscillations with subsequent fragmentation events and secondary contacts, making this an interesting study system to investigate the evolution and biogeography of Alpine clades.</p></div>","PeriodicalId":49332,"journal":{"name":"Zoologischer Anzeiger","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoologischer Anzeiger","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044523123000670","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

Subterranean fauna is an important contributor to the global fauna, but it is still understudied and a large part of its taxonomy is not yet resolved. One species complex with unresolved taxonomy is the groundwater amphipod Niphargus ruffoi, endemic to the Alpine chain. Here, we used new samples from across the Alpine arc to review the taxonomic status of the entire clade, including the species N. ruffoi and Niphargus arolaensis. We sequenced four genetic markers from the collected specimens, assessed the phylogenetic position of N. ruffoi within the genus, and studied the structure of this species complex using four molecular species delimitation methods. We tested for recombination using the alignments of the concatenated nuclear rDNA genes. The phylogenetic analyses revealed high support for the monophyly of the studied species complex, defining two lineages (i.e., N. arolaensis and N. ruffoi) within the clade. Molecular species delimitation methods suggested that N. arolaensis is a single species, while N. ruffoi should be considered as a species complex of three (using ITS) to eight (using COI) putative species. Moreover, we found a discrepancy between the different nuclear ribosomal DNA markers, indicating a possible recombination with fragments of 28S DNA of N. ruffoi s. lat. present in the genome of N. arolaensis. For the above-mentioned reasons, the internal phylogenetic structure of N. ruffoi s. lat. could not be fully resolved. Moreover, no clear morphological evidence supported the molecular species delimitation. Consequently, no taxonomic changes were proposed. We postulate that this complex scenario was influenced by Pleistocene climate oscillations with subsequent fragmentation events and secondary contacts, making this an interesting study system to investigate the evolution and biogeography of Alpine clades.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
系统发育结构和分子物种划分暗示了阿尔卑斯特有的Niphargus分支(甲壳纲,两栖纲)的复杂进化史
地下动物群是全球动物群的重要组成部分,但其研究仍然不足,其分类学的很大一部分尚未解决。一个尚未解决分类学问题的物种复合体是地下水两栖动物Niphargus ruffoi,它是阿尔卑斯山脉的特有物种。在这里,我们使用了来自阿尔卑斯弧的新样本来审查整个分支的分类状态,包括N.ruffoi和Niphargus arolaensis。我们对采集的标本中的四个遗传标记进行了测序,评估了N.ruffoi在该属中的系统发育位置,并使用四种分子物种划分方法研究了该物种复合体的结构。我们使用连接的核rDNA基因的比对来测试重组。系统发育分析显示,对所研究物种复合体的单系性有很高的支持,在分支中定义了两个谱系(即N.arolaensis和N.ruffoi)。分子物种划分方法表明,北美猪笼草是一个单一的物种,而法国猪笼草应被视为一个由三个(使用ITS)到八个(使用COI)推定物种组成的物种复合体。此外,我们发现不同的核核糖体DNA标记之间存在差异,这表明可能与北美猪笼草基因组中的28S DNA片段重组。由于上述原因,无法完全解析N.ruffois.lat.的内部系统发育结构。此外,没有明确的形态学证据支持分子物种的划界。因此,没有提出任何分类学上的改变。我们假设,这种复杂的情况受到更新世气候振荡以及随后的碎裂事件和二次接触的影响,使其成为研究阿尔卑斯山脉分支进化和生物地理学的一个有趣的研究系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Zoologischer Anzeiger
Zoologischer Anzeiger 生物-动物学
CiteScore
2.80
自引率
7.10%
发文量
75
审稿时长
>12 weeks
期刊介绍: Zoologischer Anzeiger - A Journal of Comparative Zoology is devoted to comparative zoology with a special emphasis on morphology, systematics, biogeography, and evolutionary biology targeting all metazoans, both modern and extinct. We also consider taxonomic submissions addressing a broader systematic and/or evolutionary context. The overall aim of the journal is to contribute to our understanding of the organismic world from an evolutionary perspective. The journal Zoologischer Anzeiger invites suggestions for special issues. Interested parties may contact one of the editors.
期刊最新文献
To be, or not tu-be? Population structure and connectivity in three Arctic kinorhynch species: Echinoderes aquilonius, Echinoderes eximus and Echinoderes remanei Discovery of two new species of cobia (Pisces; Rachycentridae) inhabiting the makran coast of the northern Arabian Sea with notes on their biology and ecology Kinomenclature: An analysis of scientific names of mud dragons (Kinorhyncha) Morphological differences on the mouthparts among six species of cockchafer (Coleoptera: Melolonthinae), with discussions on their feeding habits First evidence of cryptic diversity in mud dragons (kinorhyncha) and description of Echinoderes quasae sp. nov. from the northeastern pacific coast
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1