The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine

Abstract

Pickering emulsions were prepared by phacoemulsification in an ice water bath with squalene as the oil phase and an aluminum adjuvant as the particle stabilizer. The effects of formulation and process conditions on the size and distribution of the Pickering emulsions were investigated. Pickering emulsions prepared under the optimal prescription and process conditions were mixed with a peptide antigen to obtain a peptide vaccine. The optimal prescription and process condition of the Pickering emulsion is as follows: squalene as the oil phase, ultra-pure water as the water phase with 5 mg/mL aluminum adjuvant, and an ultrasonication time of 4 min at 200 W power. BALB/c mice were immunized with the peptide vaccine, and the ability of the Pickering emulsion as an immunological adjuvant to improve the efficacy of the peptide vaccine was evaluated. Under optimal conditions, a Pickering emulsion with a small particle size (430.8 nm), uniform distribution (polydispersion index of 16.9%), and zeta potential of 31.5 mV, was obtained. Immunological results showed that the serum specific antibody level in the vaccinated group reached 1×10⁴ after three immunizations. The proportion of CD4⁺T cells and CD4/CD8 cells was significantly higher (P<0.05) in the vaccinated groups than the blank control group. Further, cytokine (TNF-α) secretion decreased in the aluminum adjuvant and Pickering emulsion groups but increased in the Freund's adjuvant group. All three vaccinated groups of mice exhibited low but detectable levels of IFN-γ secretion.