{"title":"Screening of Nutrients for Enrichment of Extracellular Pullulanase Production by Isolated Bacillus cereus KKSJ1981 Using Plackett–Burman Design","authors":"Jonna Siva Jyothi, Emandi Hemalatha, Tadikamalla Satish, Kadimpati Kishore Kumar","doi":"10.1007/s40009-023-01241-y","DOIUrl":null,"url":null,"abstract":"<div><p>Current work intended to screen the different carbon, nitrogen sources and minerals which have a significant impact on the production of pullulanase by isolated <i>Bacillus cereus</i> KKSJ 1981 using Plackett–Burman design (PBD). Total 11 compounds (5 carbon sources, 3 nitrogen sources and 3 mineral salts) were screened by using 16 runs PBD. Each variable studied at 3 levels. The data were analyzed by first-order polynomial equation. The correlation coefficient was found to be 0.9876 and indicates the goodness of fit. Pareto chart of effects, normal probability plot and main effects plots were used to identify the significant variables and their level. It was observed that monosaccharide’s and dextran were found to be insignificant and remaining all variables found to be significant for production of pullulanase by isolated bacteria. Among all significant variables yeast extract has highest effect followed by soluble starch and MnSO<sub>4</sub>. This study reveals that yeast extract at low concentration, soluble starch and MnSO<sub>4</sub> at higher level needed for the high titer of pullulanase production from <i>B.cereus</i> KKSJ 1981.</p></div>","PeriodicalId":717,"journal":{"name":"National Academy Science Letters","volume":"46 3","pages":"233 - 238"},"PeriodicalIF":1.2000,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"National Academy Science Letters","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1007/s40009-023-01241-y","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Current work intended to screen the different carbon, nitrogen sources and minerals which have a significant impact on the production of pullulanase by isolated Bacillus cereus KKSJ 1981 using Plackett–Burman design (PBD). Total 11 compounds (5 carbon sources, 3 nitrogen sources and 3 mineral salts) were screened by using 16 runs PBD. Each variable studied at 3 levels. The data were analyzed by first-order polynomial equation. The correlation coefficient was found to be 0.9876 and indicates the goodness of fit. Pareto chart of effects, normal probability plot and main effects plots were used to identify the significant variables and their level. It was observed that monosaccharide’s and dextran were found to be insignificant and remaining all variables found to be significant for production of pullulanase by isolated bacteria. Among all significant variables yeast extract has highest effect followed by soluble starch and MnSO4. This study reveals that yeast extract at low concentration, soluble starch and MnSO4 at higher level needed for the high titer of pullulanase production from B.cereus KKSJ 1981.
期刊介绍：
The National Academy Science Letters is published by the National Academy of Sciences, India, since 1978. The publication of this unique journal was started with a view to give quick and wide publicity to the innovations in all fields of science
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