{"title":"Evaluation of Toxicity in Mouse Bone Marrow Progenitor Cells","authors":"Peace C. Ezeh, Huan Xu, Shu Chun Wang, Sebastian Medina, Scott W. Burchiel","doi":"10.1002/0471140856.tx1809s67","DOIUrl":null,"url":null,"abstract":"<p>Development of blood cells through hematopoiesis occurs in the bone marrow (BM), and can be adversely impacted by various substances and/or conditions ranging from known therapeutic, intentionally administered xenobiotics to unintentional food additives and exposure to environmental chemicals. The principles underlying the techniques for evaluating toxicity to BM progenitors (erythroid, myeloid, and lymphoid) exploit changes in the normal hematopoietic process, biochemical cell surface and intracellular markers, as well as components of the BM microenvironment. Toxicological investigations following in vivo exposures of mice or in vitro exposures of mouse primary BM cell cultures allow the assessment of the developmental and functional integrity of BM cells, cell population shifts, and adverse biochemical effects due to toxicity. Colony forming unit (CFU) assays and flow cytometry are indispensable techniques in these toxicity studies. © 2016 by John Wiley & Sons, Inc.</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"67 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/0471140856.tx1809s67","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/0471140856.tx1809s67","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
Development of blood cells through hematopoiesis occurs in the bone marrow (BM), and can be adversely impacted by various substances and/or conditions ranging from known therapeutic, intentionally administered xenobiotics to unintentional food additives and exposure to environmental chemicals. The principles underlying the techniques for evaluating toxicity to BM progenitors (erythroid, myeloid, and lymphoid) exploit changes in the normal hematopoietic process, biochemical cell surface and intracellular markers, as well as components of the BM microenvironment. Toxicological investigations following in vivo exposures of mice or in vitro exposures of mouse primary BM cell cultures allow the assessment of the developmental and functional integrity of BM cells, cell population shifts, and adverse biochemical effects due to toxicity. Colony forming unit (CFU) assays and flow cytometry are indispensable techniques in these toxicity studies. © 2016 by John Wiley & Sons, Inc.
小鼠骨髓祖细胞毒性评价
造血过程中血细胞的发育发生在骨髓(BM)中,并可能受到各种物质和/或条件的不利影响,这些物质和/或条件包括已知的治疗性、有意使用的异种抗生素、无意使用的食品添加剂和暴露于环境化学物质。评估骨髓基质祖细胞(红系、髓系和淋巴系)毒性的基本技术原则是利用正常造血过程、生化细胞表面和细胞内标记物以及骨髓基质微环境成分的变化。在小鼠体内暴露或小鼠原代骨髓细胞培养物体外暴露后进行毒理学研究,可以评估骨髓细胞的发育和功能完整性、细胞群变化以及由于毒性引起的不良生化效应。菌落形成单位(CFU)测定和流式细胞术是这些毒性研究中不可缺少的技术。©2016 by John Wiley &儿子,Inc。
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