Histopathological Evaluation of Skeletal Muscle with Specific Reference to Mouse Models of Muscular Dystrophy

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2016-12-01 DOI:10.1002/cpmo.19

R. Terry, D. Wells

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引用次数: 6

Abstract

The muscular dystrophies are a diverse group of degenerative diseases for which many mouse models are available. These models are frequently used to assess potential therapeutic interventions and histological evaluation of multiple muscles is an important part of this assessment. Histological evaluation is especially useful when combined with tests of muscle function. This unit describes a protocol for necropsy, processing, cryosectioning, and histopathological evaluation of murine skeletal muscles, which is applicable to both models of muscular dystrophy and other neuromuscular conditions. Key histopathological features of dystrophic muscle are discussed using the mdx mouse (a model of Duchenne muscular dystrophy) as an example. Optimal handling during dissection, processing and sectioning is vital to avoid artifacts that can confound or prevent future analyses. Muscles carefully processed using this protocol are suitable for further evaluation using immunohistochemistry, immunofluorescence, special histochemical stains, and immuoblotting. © 2016 by John Wiley & Sons, Inc.

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肌肉萎缩症小鼠模型骨骼肌的组织病理学评价

肌肉萎缩症是一组不同的退行性疾病，许多小鼠模型是可用的。这些模型经常用于评估潜在的治疗干预措施，多块肌肉的组织学评估是该评估的重要组成部分。组织学评估与肌肉功能测试相结合尤其有用。本单元描述了小鼠骨骼肌的尸检、处理、冷冻切片和组织病理学评估的方案，适用于肌肉萎缩症模型和其他神经肌肉疾病。以mdx小鼠(杜氏肌营养不良模型)为例，讨论了肌营养不良的主要组织病理学特征。在解剖，加工和切片过程中的最佳处理对于避免可能混淆或阻止未来分析的伪影至关重要。使用该方案仔细处理的肌肉适合使用免疫组织化学、免疫荧光、特殊组织化学染色和免疫印迹进行进一步评估。©2016 by John Wiley & Sons, Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.