Observation of the compatibility of stimulated Raman histology with pathology workflow and genome sequencing

Abstract

We show that human samples imaged with stimulated Raman histology (SRH) can be successfully used in a typical pathology workflow including hematoxylin and eosin staining (HE), immunohistochemistry, and genome sequencing. We provide a morphological comparison of SRH images with the gold standard HE and HES (HE and saffron) staining over three normal tissues from various organs (liver, kidney, and ileum) and two tumoral brain samples (metastatic adenocarcinoma and glioblastoma). Most importantly, we show that a sample imaged with SRH has a comparable microscopic appearance as a control sample (that was not imaged by SRH) including protein expression evaluated by immunohistochemistry as well as similar genetic alterations evaluated by genome sequencing. This suggests that SRH can provide a direct diagnosis without material preparation nor consumption.