Research Progress and Application of Bioorthogonal Reactions in Biomolecular Analysis and Disease Diagnosis

IF 8.6 2区化学 Q1 Chemistry Topics in Current Chemistry Pub Date : 2021-09-29 DOI:10.1007/s41061-021-00352-8

Zilong Li, Qinhua Chen, Jin Wang, Xiaoyan Pan, Wen Lu

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引用次数: 5

Abstract

Bioorthogonal reactions are rapid, specific and high yield reactions that can be performed in in vivo microenvironments or simulated microenvironments. At present, the main biorthogonal reactions include Staudinger ligation, copper-catalyzed azide alkyne cycloaddition, strain-promoted [3 + 2] reaction, tetrazine ligation, metal-catalyzed coupling reaction and photo-induced biorthogonal reactions. To date, many reviews have reported that bioorthogonal reactions have been used widely as a powerful tool in the field of life sciences, such as in target recognition, drug discovery, drug activation, omics research, visualization of life processes or exogenous bacterial infection processes, signal transduction pathway research, chemical reaction dynamics analysis, disease diagnosis and treatment. In contrast, to date, few studies have investigated the application of bioorthogonal reactions in the analysis of biomacromolecules in vivo. Therefore, the application of bioorthogonal reactions in the analysis of proteins, nucleic acids, metabolites, enzyme activities and other endogenous molecules, and the determination of disease-related targets is reviewed. In addition, this review discusses the future development opportunities and challenges of biorthogonal reactions.

Graphic Abstract

This review presents an overview of recent advances for application in biomolecular analysis and disease diagnosis, with a focus on proteins, metabolites and RNA detection.

Abstract Image

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生物正交反应在生物分子分析和疾病诊断中的研究进展及应用

生物正交反应是一种快速、特异、产率高的反应，可在体内微环境或模拟微环境中进行。目前，双正交反应主要有Staudinger连接反应、铜催化叠氮化炔烃环加成反应、菌株促进[3 + 2]反应、四嗪连接反应、金属催化偶联反应和光诱导双正交反应。迄今为止，许多综述报道了生物正交反应作为生命科学领域的有力工具，在靶标识别、药物发现、药物活化、组学研究、生命过程或外源细菌感染过程可视化、信号转导途径研究、化学反应动力学分析、疾病诊断和治疗等领域得到了广泛的应用。相比之下，迄今为止，很少有研究将生物正交反应应用于生物大分子的体内分析。因此，对生物正交反应在蛋白质、核酸、代谢物、酶活性等内源性分子分析以及疾病相关靶点测定中的应用进行综述。此外，本文还讨论了双正交反应的未来发展机遇和挑战。本文综述了近年来在生物分子分析和疾病诊断中的应用进展，重点介绍了蛋白质、代谢物和RNA检测。

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来源期刊

Topics in Current Chemistry 化学-化学综合

CiteScore

11.70

自引率

1.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Topics in Current Chemistry provides in-depth analyses and forward-thinking perspectives on the latest advancements in chemical research. This renowned journal encompasses various domains within chemical science and their intersections with biology, medicine, physics, and materials science. Each collection within the journal aims to offer a comprehensive understanding, accessible to both academic and industrial readers, of emerging research in an area that captivates a broader scientific community. In essence, Topics in Current Chemistry illuminates cutting-edge chemical research, fosters interdisciplinary collaboration, and facilitates knowledge-sharing among diverse scientific audiences.