Agnes Beenfeldt Petersen, Idd Andrea Christensen, Mette E. Rønne, Emil G. P. Stender, David Teze, Birte Svensson, Finn Lillelund Aachmann
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引用次数: 0
Abstract
To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the 1H, 13C, and 15N NMR chemical shift assignment for BeKdgF is reported.
为了充分利用海藻生物量中的碳水化合物，必须了解海藻酸盐多糖家族的降解。海藻酸盐降解的一个步骤是由酶KdgF催化的4,5-不饱和单醛酸盐转化为4-脱氧- l -红-5-己糖糖。在这项研究中，来自人类肠道微生物群的蛋氏拟杆菌的BeKdgF在大肠杆菌中被同位素标记。这里报告了BeKdgF的1H, 13C和15N核磁共振化学位移分配。
期刊介绍：
Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties.
Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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