Susan C. Morpeth , Jim F. Huggett , David R. Murdoch , J. Anthony G. Scott
{"title":"Making standards for quantitative real-time pneumococcal PCR","authors":"Susan C. Morpeth , Jim F. Huggett , David R. Murdoch , J. Anthony G. Scott","doi":"10.1016/j.bdq.2014.11.003","DOIUrl":null,"url":null,"abstract":"<div><p>Quantitative <em>lytA</em> PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of <em>Streptococcus pneumoniae</em> by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.</p></div>","PeriodicalId":38073,"journal":{"name":"Biomolecular Detection and Quantification","volume":"2 ","pages":"Pages 1-3"},"PeriodicalIF":0.0000,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bdq.2014.11.003","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomolecular Detection and Quantification","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214753514000114","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 8
Abstract
Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.
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