Effects of castration on the expression of aquaporins and apoptosis in the ventral prostate

IF 0.6 4区医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Journal of Mens Health Pub Date : 2012-12-01 DOI:10.1016/j.jomh.2012.03.015

Seung-Il Jung MD, PhD , Hyun-Suk Lee MS , Hyung-Seok Kim MD, PhD , Kyuyoun Ahn MD, PhD , Kwangsung Park MD, PhD

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引用次数: 2

Abstract

Background

Aquaporins (AQPs) are transmembrane proteins that play a major role in the regulation of membrane water permeability. The purpose of this study was to determine the expression and localization of AQPs in the ventral prostate (VP) and to predict a possible role for AQPs in apoptotic rat VP, as there has been little research on the expression of AQPs in rat prostate.

Methods

Male Sprague–Dawley rats were divided into two groups: a control group and a castration group. The expression and cellular localization of AQPs 1–9 in the VP were determined using immunochemistry and Western blot analysis, respectively. Apoptosis of the VP was determined using Western blot analysis of Bax and Bcl-2 proteins.

Results

Among the AQPs, AQP1 and 9 were expressed in rat VP. AQP9 expression was localized on the epithelial cell membranes in the intermediate region, which has a secretary function. The expression of AQP1 was not changed after castration; however, the expression of AQP9 was significantly decreased. Apoptosis of the epithelium developed on day 1 after castration and reached a maximum on day 3. At that time, the expression of AQP9 was highly preserved.

Conclusions

In rat VP, only AQP9 was expressed on the epithelial cell membrane in the intermediate region, which has a secretary function, implying that AQP9 may play a role in secretory processes. The apoptotic process and AQP9 expression was correlated, so further study of the role of AQPs in castration-induced apoptotic and transition processes will be required.

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去势对腹侧前列腺水通道蛋白表达及细胞凋亡的影响

水通道蛋白(AQPs)是一种跨膜蛋白，在调节细胞膜透水性中起重要作用。本研究的目的是确定AQPs在腹侧前列腺(VP)中的表达和定位，并预测AQPs在大鼠VP凋亡中的可能作用，因为目前关于AQPs在大鼠前列腺中的表达的研究很少。方法将sd - dawley大鼠分为对照组和去势组。免疫化学和Western blot检测AQPs 1-9在VP中的表达和细胞定位。Bax和Bcl-2蛋白Western blot检测VP的凋亡情况。结果aqp中AQP1和aq9在大鼠VP中表达。AQP9的表达定位于上皮细胞膜中间区域，具有秘书功能。去势后AQP1表达无明显变化;而AQP9的表达明显降低。上皮细胞凋亡在去势后第1天开始出现，在第3天达到最大。此时，AQP9的表达被高度保存。结论在大鼠VP中，只有AQP9在上皮细胞膜中间区域表达，具有分泌功能，提示AQP9可能在分泌过程中发挥作用。凋亡过程与AQP9表达相关，因此需要进一步研究AQPs在去势诱导的细胞凋亡和凋亡转化过程中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Mens Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

0.90

自引率

28.60%

发文量

153

审稿时长

>12 weeks

期刊介绍： JOMH is an international, peer-reviewed, open access journal. JOMH publishes cutting-edge advances in a wide range of diseases and conditions, including diagnostic procedures, therapeutic management strategies, and innovative clinical research in gender-based biology. It also addresses sexual disparities in health, life expectancy, lifestyle and behaviors and so on. Scientists are encouraged to publish their experimental, theoretical, and descriptive studies and observations in as much detail as possible.