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Factors associated with knowledge, confidence, self-efficacy, and satisfaction in African American men's decisions about prostate cancer screening. 非裔美国男性前列腺癌筛查决策中与知识、自信、自我效能和满意度相关的因素
IF 0.6 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2025-01-01 Epub Date: 2025-01-30
Margarita Echeverri, Kyazia Felder, David Anderson, Elora Apantaku

Background: African American men (AAM) have persistently had the highest incidence and mortality rates for prostate cancer (PrCa) in the United States. Considering that current guidelines recommend the practice of shared decision-making to determine whether or not to undergo a Prostate-Specific Antigen (PSA) test for the early detection of PrCa, this study focuses on the identification of key factors influencing AAM decisions regarding having or not having PSA screenings.

Methods: Cross-sectional study of 152 AAM who completed study surveys. Statistical analysis included frequencies, means, and distributions and methods to test for differences in knowledge confidence, satisfaction and, self-efficacy when making decisions.

Results: 94% of participants would get a PSA test if offered, only 57% knew that the PSA test is a blood test. Participants who reported having had a PSA test before the baseline had significantly higher mean scores than their counterparts in the knowledge about the definition of the PSA and biopsy exams (p = 0.04), and in the confidence (p = 0.005) and efficacy (p = 0.002) scales when making PSA screening decisions. Older participants were more likely to have had a PSA test (p < 0.0001) and to intend to screen (p = 0.0441).

Conclusions: Significant differences in the satisfaction scale by clinical site (p < 0.001) may underscore the influence of clinicians' practices in participants' satisfaction with their decisions. Results suggest that patients' experience of care has the potential to positively influence PSA screening. It is our call that type of health insurance, knowledge about PrCa and PSA, and having had a PSA test in the past, as well as the patient's characteristics (age, race and family history of PrCa) be considered when discussing with patients the harms/benefits of PSA screening and their preferences to have or not have the PSA test.

背景:非裔美国男性(AAM)一直是美国前列腺癌(PrCa)发病率和死亡率最高的人群。考虑到目前的指南推荐共同决策的做法,以确定是否进行前列腺特异性抗原(PSA)检测,以早期检测PrCa,本研究的重点是确定影响AAM决定是否进行PSA筛查的关键因素。方法:对152例完成研究调查的AAM进行横断面研究。统计分析包括频率、均值、分布和方法,以检验在决策时知识自信、满意度和自我效能的差异。结果:如果提供PSA测试,94%的参与者会接受,只有57%的人知道PSA测试是一种血液测试。报告在基线之前进行过PSA测试的参与者在PSA和活检检查的定义知识(p = 0.04)以及在制定PSA筛查决策时的置信度(p = 0.005)和有效性(p = 0.002)量表上的平均得分明显高于同行。年龄较大的参与者更有可能进行PSA测试(p < 0.0001),并打算进行筛查(p = 0.0441)。结论:临床地点满意度量表的显著差异(p < 0.001)可能强调了临床医生的实践对参与者决策满意度的影响。结果表明,患者的护理经历有可能对PSA筛查产生积极影响。我们呼吁,在与患者讨论PSA筛查的危害/益处以及他们是否选择进行PSA检测时,应考虑健康保险类型、对PrCa和PSA的了解、过去是否进行过PSA检测,以及患者的特征(年龄、种族和PrCa家族史)。
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引用次数: 0
Methods and Efficacy of Processing Testicular Sperm Samples in Obstructive and Non-Obstructive Azoospermia: A Systematic Review. 梗阻性和非梗阻性无精子症睾丸精子标本处理方法及疗效综述。
IF 0.6 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-11-01 Epub Date: 2024-11-29 DOI: 10.22514/jomh.2024.182
Terrence C Tsou, Shagnik Ray, Mahir Maruf, Taylor P Kohn, Mohammad H Zaman, Michael F Ayenew, Arvin K George, Amin S Herati

Intracytoplasmic sperm injection (ICSI) is a cornerstone in managing male infertility, especially in obstructive azoospermia (OA) and non-obstructive azoospermia (NOA), necessitating sperm retrieval via testicular sperm extraction (TESE) or microdissection TESE (mTESE). However, the varied post-sperm extraction processing methods pose uncertainty regarding optimal approaches. To address this, a systematic review following preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) guidelines was conducted, identifying 16 relevant studies. These studies exhibited significant heterogeneity in methodologies and outcomes, with mechanical preparation and enzymatic digestion being the primary techniques investigated. Mechanical methods, including shredding, mincing, vortexing, and crushing, yielded varying sperm counts per 100 mg of tissue, with mincing showing promise in NOA cases. Enzymatic digestion, particularly with collagenase type IV, also showed effectiveness, though inconsistently. Additionally, techniques such as microfluidics and magnetic levitation showed potential for improving sperm retrieval efficiency. However, the lack of standardization in outcomes and reporting impedes the establishment of best practice protocols. While collagenase type IV with elastase seemed promising for OA samples and microfluidics for NOA cases, further studies with standardized methodologies and outcomes are necessary. Assessment of DNA damage and comparisons of ICSI success rates between processing methods are crucial for informed clinical practice. In conclusion, optimizing sperm quantity and quality for ICSI necessitates standardized methodologies and outcomes, with microfluidics and collagenase type IV with elastase showing promise pending further validation through well-designed studies.

卵胞浆内单精子注射(ICSI)是治疗男性不育症的基石,特别是在阻塞性无精子症(OA)和非阻塞性无精子症(NOA)中,需要通过睾丸精子提取(TESE)或显微解剖TESE (mTESE)来获取精子。然而,各种精子提取后处理方法对最佳方法构成不确定性。为了解决这个问题,根据系统评价和荟萃分析方案(PRISMA-P)指南的首选报告项目进行了系统评价,确定了16项相关研究。这些研究在方法和结果上表现出显著的异质性,机械制备和酶消化是研究的主要技术。机械方法,包括切碎、绞碎、涡流和粉碎,每100毫克组织产生不同的精子数量,绞碎在NOA病例中显示出希望。酶消化,特别是ⅳ型胶原酶,也显示出有效性,尽管不一致。此外,微流体和磁悬浮等技术显示出提高精子回收效率的潜力。然而,结果和报告缺乏标准化阻碍了最佳实践方案的建立。虽然IV型胶原酶与弹性酶似乎有希望用于OA样品和NOA病例的微流体,但有必要进一步研究标准化的方法和结果。评估DNA损伤和比较处理方法之间的ICSI成功率对知情的临床实践至关重要。总之,优化ICSI的精子数量和质量需要标准化的方法和结果,微流体和IV型胶原酶结合弹性酶显示出希望,有待通过精心设计的研究进一步验证。
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引用次数: 0
Exploring hypermasculinity as a moderator between sexual violence victimization and adverse mental health effects among sentenced incarcerated men. 探讨在被判刑监禁的男性中,过度男性化在性暴力受害与不良心理健康影响之间的调节作用。
IF 0.6 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-10-01 Epub Date: 2024-10-30 DOI: 10.22514/jomh.2024.169
Sebenzile Nkosi, Kopano Ratele, Yandisa Sikweyiya, Neo Morojele

Sexual assault is a common occurrence among incarcerated men in correctional facilities, and severely impacts the psychological well-being of the victim. We explored hypermasculinity as a moderator between sexual violence (SV) victimization and consequent mental health outcomes (prototypic and masculine depression, alcohol use and drug use) among incarcerated men in Tshwane, South Africa. A convenience sample of 160 incarcerated men self-completed two questionnaires: Questionnaire 1, completed during their incarceration, included themes such as demographics, incarceration-related characteristics, and involvement in physical fighting, and Questionnaire 2, completed at least two months post their release from incarceration, included themes such as demographics, gang affiliation, hypermasculinity, SV victimization, misuse of substances and/or alcohol, prototypic depression and masculine depression. Descriptive analyses were conducted to examine sample characteristics. Associations between variables were examined with Pearson's correlational analysis while the moderation effect was tested with the SPSS PROCESS macro software. Hypermasculinity had a significant moderation effect on SV victimization and prototypic depression. Post hoc probing analyses showed a significant negative relationship between SV victimization and prototypic depression among men who highly endorsed hypermasculinity, but no significant relationship was observed among those who endorsed hypermasculinity at low or average levels. The moderation effect of hypermasculinity was not statistically significant for SV victimization and masculine depression, alcohol use and drug use. Men who were high in hypermasculinity had a decreased likelihood of prototypic depression despite having been sexually victimized. Interventions that address SV-related mental health outcomes should take into account the role of masculine ideals in SV-related psychological reactions among incarcerated men or men with a history of incarceration.

性侵犯在监狱服刑人员中很常见,严重影响受害者的心理健康。我们探讨了在南非Tshwane被监禁的男性中,过度男子气概作为性暴力(SV)受害者和随之而来的精神健康结果(原型和男性抑郁症、酒精使用和药物使用)之间的调节因素。为了方便起见,160名在押男子自行完成了两份问卷:问卷1是在监禁期间完成的,包括人口统计、监禁相关特征和参与身体战斗等主题;问卷2是在他们出狱至少两个月后完成的,包括人口统计、帮派关系、过度男性化、性侵犯受害者、滥用药物和/或酒精、典型抑郁症和男性抑郁症等主题。进行描述性分析以检验样本特征。变量间的相关性采用Pearson相关分析检验,调节效应采用SPSS PROCESS宏观软件检验。高男子气概对性侵犯受害和原型抑郁有显著的调节作用。事后探查分析显示,在高度认可超级男子气概的男性中,SV受害与原型抑郁之间存在显著的负相关,但在那些认可低水平或平均水平的超级男子气概的男性中,没有观察到显著的关系。高男子气概对性侵犯受害、男性抑郁、酒精使用和药物使用的调节作用无统计学意义。高阳刚之气的男性尽管遭受过性侵害,但患原型抑郁症的可能性较低。针对性行为相关心理健康结果的干预措施应考虑到男性理想在监禁男性或有监禁史男性的性行为相关心理反应中的作用。
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引用次数: 0
Video-endoscopic inguinal lymphadenectomy (VEIL) oncological and surgical benefits compared to open inguinal lymph node dissection (ILND). 与开放式腹股沟淋巴结清扫术(ILND)相比,视频内镜下腹股沟淋巴结切除术(VEIL)在肿瘤和手术方面的益处。
IF 0.6 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-07-01 Epub Date: 2024-07-30 DOI: 10.22514/jomh.2024.106
Fayssal Alqudrah, Rachel Passarelli, Jennifer Sykes, Raeesa Islam, Kevin Chua, Saum Ghodoussipour

Penile cancer accounts for about 1% of all male cancer diagnoses in the United States. Regional lymph node involvement is strongly correlated with overall outcomes, and as such the procedure of inguinal lymph node dissection (ILND) is imperative to the diagnostic and oncologic outcomes for these patients. The current gold standard of open ILND presents challenges in mitigating detrimental postoperative sequalae such as wound complications and lymphedema, without compromising oncologic outcomes. There has been a growing interest and shift in minimally invasive (MIS) approaches to tackle the challenges seen in the open approach to ILND. Several different minimally invasive techniques such as laparoscopic video-endoscopic inguinal lymphadenectomy (VEIL) and robotic-assisted VEIL approaches have been explored and described in the literature. A systematic literature search of PubMed and Medline (OVID) literature review was performed to assess outcomes in MIS approaches to ILND in comparison to traditional open approach. Key words included penile cancer/penile neoplasms, minimally-invasive procedures, robotics, video-endoscopic, robotic-assisted, inguinal lymphadenectomy, and inguinal lymph node dissections. Studies show that MIS approaches to ILND have potential to reduce high-grade postoperative complications, operative time, and hospital stay while ensuring oncologic outcomes. Despite the learning curve associated with MIS ILND, preliminary data does suggest favorable outcomes. Prospective, randomized trials are needed to reveal the full benefit of MIS ILND compared to open ILND.

在美国,阴茎癌约占所有男性癌症诊断的1%。局部淋巴结受累与总体预后密切相关,因此腹股沟淋巴结清扫(ILND)手术对这些患者的诊断和肿瘤预后至关重要。目前的开放式ILND金标准在不影响肿瘤预后的情况下,在减轻有害的术后后遗症(如伤口并发症和淋巴水肿)方面提出了挑战。人们对微创(MIS)方法的兴趣和转变越来越大,以解决开放式方法治疗ILND所面临的挑战。几种不同的微创技术,如腹腔镜-内镜腹股沟淋巴结切除术(VEIL)和机器人辅助的VEIL方法已经在文献中进行了探索和描述。对PubMed和Medline (OVID)文献综述进行了系统的文献检索,以评估MIS方法与传统开放方法相比治疗ILND的结果。关键词:阴茎癌/阴茎肿瘤,微创手术,机器人,视频内镜,机器人辅助,腹股沟淋巴结切除术,腹股沟淋巴结清扫。研究表明,MIS入路治疗ILND具有减少高度术后并发症、手术时间和住院时间的潜力,同时确保肿瘤预后。尽管与MIS ILND相关的学习曲线,初步数据确实显示出有利的结果。需要前瞻性的随机试验来揭示MIS ILND与开放式ILND相比的全部益处。
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引用次数: 0
Exploration of experiences and attitudes associated with lung health promotion among Black males with a history of smoking. 探讨有吸烟史的黑人男性在肺部健康宣传方面的经验和态度。
IF 0.6 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-01-01 Epub Date: 2024-01-29 DOI: 10.22514/jomh.2024.005
Alicia K Matthews, Suchanart Inwanna, Dami Oyaluade, Alexis Chappel, Jennifer Akufo, Sage J Kim, Rohan Jeremiah

To examine knowledge and attitudes about lung health promotion (smoking cessation and lung cancer screening) among Black male smokers in a large Midwestern city in the United States. Semi-structured, in-depth interviews were conducted with 25 study participants. Each interview lasted approximately 45 minutes. Participants also completed a brief (5-10 minutes) survey measuring demographic characteristics, smoking experiences and knowledge and attitudes about lung health promotion activities. Descriptive statistics were used for quantitative data, and deductive thematic analysis for qualitative data analysis. The mean age of study participants was 57.5 years. Eighty-four percent of participants were current smokers, with the majority being daily smokers. Perceived risk for lung cancer was mixed, with 56% of participants endorsing that they considered themselves to be at high or moderate risk and the remaining 44% at low or no risk for lung cancer. Forty percent of participants reported having had a test to check their lungs for cancer. Participants were aware of the health risks associated with smoking but reported limited assistance from providers regarding the receipt of smoking cessation treatments. Awareness of lung cancer screening was limited, but participants expressed openness to screening; however, barriers were anticipated, including costs, fear and a reduced willingness to be screened in the absence of symptoms. Study participants reported limited experiences with lung health promotion activities. Knowledge about the facilitators and barriers can be used to develop health promotion interventions targeting smoking cessation and lung cancer screening.

研究美国中西部某大城市黑人男性吸烟者对促进肺部健康(戒烟和肺癌筛查)的认识和态度。对 25 名研究参与者进行了半结构化深入访谈。每次访谈持续约 45 分钟。参与者还完成了一项简短(5-10 分钟)的调查,调查内容包括人口特征、吸烟经历以及对肺健康促进活动的认识和态度。定量数据采用描述性统计,定性数据采用演绎主题分析。研究参与者的平均年龄为 57.5 岁。84%的参与者目前是吸烟者,其中大多数是日常吸烟者。对肺癌风险的认识不一,56%的参与者认为自己有高风险或中度风险,其余44%的参与者认为自己有低风险或无风险。40%的参与者表示曾接受过肺癌检查。参与者意识到吸烟对健康的危害,但表示在接受戒烟治疗方面,医疗服务提供者提供的帮助有限。参与者对肺癌筛查的认识有限,但表示对筛查持开放态度;不过,预计会遇到一些障碍,包括费用、恐惧以及在没有症状的情况下接受筛查的意愿降低。研究参与者报告称,他们在肺部健康促进活动方面的经验有限。有关促进因素和障碍的知识可用于制定针对戒烟和肺癌筛查的健康促进干预措施。
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引用次数: 0
Patient education services and the organizational factors affecting them at teaching hospitals affiliated with Mashhad University of Medical Sciences (MUMS), 2008 马什哈德医科大学附属教学医院的病人教育服务和影响他们的组织因素,2008年
IF 0.7 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2012-12-01 DOI: 10.1016/j.jomh.2012.04.004
Ali Vafaee-Najar , Hossein Ebrahimipour , Mohammad-Reza Shidfar , Reza Khani-Jazani

Background

Presenting educational and communicational programs for patients is considered to be one of the most significant executive roles of hospitals. Studying the status of patients’ training services, identifying the barriers, and the organizational factors that affect these in the hospitals affiliated to the Mashhad University of Medical Sciences (MUMS) was the aim of this study.

Method

This was a descriptive cross-sectional study. The participants were 441 patients, 200 physicians, 185 nurses, and 70 supervisors as well as the managers of the MUMS hospitals during the year 2008. Data were gathered using questionnaires and interviews. SPSS software (version 2), and ANOVA were used to analyze the data.

Results

Only 42% of patients received the necessary training and 94% of them were not satisfied with the amount of training received (which was less than 5 min in 83.3% of cases) or with the content (i.e. mainly related to the method of treating the disease). Of the physicians, 79.4% considered lack of time to be the most important barrier to patient’ training and 72% of the hospital managers ruled out the possibility of role play training by nurses under current conditions.

Conclusions

Underestimating the role of nurses in patient education by the patients themselves and barriers such as high workload, lack of suitable space, lack of appropriate educational materials, and lack of emphasis by managers, were the most influential factors in terms of patient training. Therefore, reviewing the job description for staff, developing retraining programs for medical staff, boosting educational facilities and encouraging the provision of patient training as part of current hospital services could have an influence on promoting staff capability in the patient education process.

为患者提供教育和交流项目被认为是医院最重要的行政职责之一。本研究的目的是研究马什哈德医科大学附属医院的患者培训服务状况,确定障碍,以及影响这些服务的组织因素。方法采用描述性横断面研究。2008年期间,参与者是441名病人、200名医生、185名护士和70名主管以及妇产联合医院的管理人员。通过问卷调查和访谈收集数据。采用SPSS软件(version 2)和方差分析(ANOVA)对数据进行分析。结果只有42%的患者接受了必要的培训,94%的患者对培训的时间不满意(83.3%的患者培训时间小于5分钟)或培训的内容不满意(即主要与治疗疾病的方法有关)。79.4%的医生认为缺乏时间是患者培训的最大障碍,72%的医院管理人员排除了护士在目前情况下进行角色扮演培训的可能性。结论患者自我低估护士在患者教育中的作用、工作量大、缺乏合适的空间、缺乏合适的教育材料、管理者不重视等障碍是影响患者培训的主要因素。因此,检讨工作人员的职位描述,为医务人员制定再培训计划,增加教育设施,并鼓励提供病人培训,作为现有医院服务的一部分,可以对提高工作人员在病人教育过程中的能力产生影响。
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引用次数: 15
“No jobs, more crime. More jobs, less crime”: structural factors affecting the health of Latino men in Detroit “没有工作,更多的犯罪。“更多的工作,更少的犯罪”:影响底特律拉丁裔男性健康的结构性因素
IF 0.7 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2012-12-01 DOI: 10.1016/j.jomh.2012.03.007
William D. Lopez MPH , Louis F. Graham DrPH, MPH , Caitlin Reardon BA , Armando Matiz Reyes DDS , Angela Reyes MPH , Mark Padilla PhD, MPH

Background

Latinos comprise 14% of the United States (U.S.) population, and approximately 75% of Latinos in the U.S. are immigrants or children of immigrants. Urban Latino men face a distinct set of structural barriers that may influence their health and well-being. The collapse of the automobile industry in Detroit, Michigan, contributed to increased rates of racially stratified unemployment.

Method

Through semi-structured interviews and ethnography, we present a case study of nine Latino men who face decreasing opportunities for formal employment and navigate the constant threat of violence and deportation in the face of Detroit's rapid deindustrialization.

Results

In light of scarce work opportunities, participants sought income from the informal labor sector. This work was often illegal or dangerous, such as drug dealing, and perpetuated a cycle of violence. The threat of violence and deportation limited mobility and contact with authorities, especially among undocumented participants.

Conclusion

In order to promote the health of urban Latino men, a careful consideration of the structural factors that shape their lives is essential. Interventions should work to leverage an extensive skill set and drive to work into meaningful formal employment, and consider the overarching effects of documentation on social mobility.

拉丁美洲人占美国人口的14%,美国大约75%的拉丁美洲人是移民或移民子女。城市拉丁裔男子面临着可能影响其健康和福祉的一系列独特的结构性障碍。密歇根州底特律市汽车工业的崩溃导致了种族失业率的上升。方法:通过半结构化访谈和民族志,我们对九名拉丁裔男性进行了案例研究,他们面临着正式就业机会的减少,面对底特律快速去工业化的持续暴力和驱逐的威胁。结果由于工作机会稀缺,参与者从非正规劳动部门寻求收入。这种工作通常是非法的或危险的,例如毒品交易,并使暴力循环永久化。暴力和驱逐的威胁限制了流动性和与当局的联系,特别是无证件参与者。结论为了促进拉丁裔城市男性的健康,必须仔细考虑影响其生活的结构性因素。干预措施应努力利用广泛的技能组合,推动工作进入有意义的正式就业,并考虑证件对社会流动的总体影响。
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引用次数: 19
Effects of castration on the expression of aquaporins and apoptosis in the ventral prostate 去势对腹侧前列腺水通道蛋白表达及细胞凋亡的影响
IF 0.7 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2012-12-01 DOI: 10.1016/j.jomh.2012.03.015
Seung-Il Jung MD, PhD , Hyun-Suk Lee MS , Hyung-Seok Kim MD, PhD , Kyuyoun Ahn MD, PhD , Kwangsung Park MD, PhD

Background

Aquaporins (AQPs) are transmembrane proteins that play a major role in the regulation of membrane water permeability. The purpose of this study was to determine the expression and localization of AQPs in the ventral prostate (VP) and to predict a possible role for AQPs in apoptotic rat VP, as there has been little research on the expression of AQPs in rat prostate.

Methods

Male Sprague–Dawley rats were divided into two groups: a control group and a castration group. The expression and cellular localization of AQPs 1–9 in the VP were determined using immunochemistry and Western blot analysis, respectively. Apoptosis of the VP was determined using Western blot analysis of Bax and Bcl-2 proteins.

Results

Among the AQPs, AQP1 and 9 were expressed in rat VP. AQP9 expression was localized on the epithelial cell membranes in the intermediate region, which has a secretary function. The expression of AQP1 was not changed after castration; however, the expression of AQP9 was significantly decreased. Apoptosis of the epithelium developed on day 1 after castration and reached a maximum on day 3. At that time, the expression of AQP9 was highly preserved.

Conclusions

In rat VP, only AQP9 was expressed on the epithelial cell membrane in the intermediate region, which has a secretary function, implying that AQP9 may play a role in secretory processes. The apoptotic process and AQP9 expression was correlated, so further study of the role of AQPs in castration-induced apoptotic and transition processes will be required.

水通道蛋白(AQPs)是一种跨膜蛋白,在调节细胞膜透水性中起重要作用。本研究的目的是确定AQPs在腹侧前列腺(VP)中的表达和定位,并预测AQPs在大鼠VP凋亡中的可能作用,因为目前关于AQPs在大鼠前列腺中的表达的研究很少。方法将sd - dawley大鼠分为对照组和去势组。免疫化学和Western blot检测AQPs 1-9在VP中的表达和细胞定位。Bax和Bcl-2蛋白Western blot检测VP的凋亡情况。结果aqp中AQP1和aq9在大鼠VP中表达。AQP9的表达定位于上皮细胞膜中间区域,具有秘书功能。去势后AQP1表达无明显变化;而AQP9的表达明显降低。上皮细胞凋亡在去势后第1天开始出现,在第3天达到最大。此时,AQP9的表达被高度保存。结论在大鼠VP中,只有AQP9在上皮细胞膜中间区域表达,具有分泌功能,提示AQP9可能在分泌过程中发挥作用。凋亡过程与AQP9表达相关,因此需要进一步研究AQPs在去势诱导的细胞凋亡和凋亡转化过程中的作用。
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引用次数: 2
Detection and analysis of MMP-2 and MMP-9 in seminal plasma 精浆中MMP-2和MMP-9的检测与分析
IF 0.7 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2012-12-01 DOI: 10.1016/j.jomh.2012.07.002
Jiu-wei Du , Ke-yi Xu , Li-yi Fang , Xin-Lan Qi

Background

The aim of this study was to explore the association between semen parameters and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in human seminal plasma samples.

Methods

Conventional semen analysis was performed on semen samples (n = 64). MMP-2 and MMP-9 expression in seminal plasma was determined using gelatin zymography.

Results

Compared to semen samples with a normal sperm count, semen samples with a low sperm count (≤ 19 × 106/ml) showed reduced sperm viability, a reduced percentage of Grade A sperm, a reduced percentage of morphologically-normal sperm, and lower proMMP-9 and MMP-9 but higher proMMP-2 and MMP-2 levels (P < 0.05). There were correlations between MMP-2 and MMP-9 expression and the percentage of Grade A sperm and morphologically-normal sperm (P < 0.05).

Conclusions

MMP-2 and MMP-9 are both present in human semen, and low-concentration semen samples have increased MMP-2 and decreased MMP-9. We also observed relationships between MMP-2, MMP-9 and seminal parameters.

本研究旨在探讨精液参数与人精浆中基质金属蛋白酶-2 (MMP-2)和基质金属蛋白酶-9 (MMP-9)表达的关系。方法对64份精液样本进行常规精液分析。明胶酶谱法测定精浆中MMP-2和MMP-9的表达。结果与正常精子数量相比,低精子数量(≤19 × 106/ml)的精子活力降低,a级精子比例降低,形态正常精子比例降低,proMMP-9和MMP-9水平降低,而proMMP-2和MMP-2水平升高(P <0.05)。MMP-2和MMP-9的表达与A级精子和形态正常精子的比例(P <0.05)。结论smmp -2和MMP-9均存在于人精液中,低浓度精液中MMP-2升高,MMP-9降低。我们还观察了MMP-2、MMP-9与种子参数之间的关系。
{"title":"Detection and analysis of MMP-2 and MMP-9 in seminal plasma","authors":"Jiu-wei Du ,&nbsp;Ke-yi Xu ,&nbsp;Li-yi Fang ,&nbsp;Xin-Lan Qi","doi":"10.1016/j.jomh.2012.07.002","DOIUrl":"10.1016/j.jomh.2012.07.002","url":null,"abstract":"<div><h3>Background</h3><p>The aim of this study was to explore the association between semen parameters and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in human seminal plasma samples.</p></div><div><h3>Methods</h3><p><span>Conventional semen analysis was performed on semen samples (</span><em>n</em> <!-->=<!--> <span>64). MMP-2 and MMP-9 expression in seminal plasma was determined using gelatin zymography.</span></p></div><div><h3>Results</h3><p>Compared to semen samples with a normal sperm count, semen samples with a low sperm count (≤ 19<!--> <!-->×<!--> <!-->10<sup>6</sup>/ml) showed reduced sperm viability, a reduced percentage of Grade A sperm, a reduced percentage of morphologically-normal sperm, and lower proMMP-9 and MMP-9 but higher proMMP-2 and MMP-2 levels (<em>P</em> <!-->&lt;<!--> <!-->0.05). There were correlations between MMP-2 and MMP-9 expression and the percentage of Grade A sperm and morphologically-normal sperm (<em>P</em> <!-->&lt;<!--> <!-->0.05).</p></div><div><h3>Conclusions</h3><p>MMP-2 and MMP-9 are both present in human semen, and low-concentration semen samples have increased MMP-2 and decreased MMP-9. We also observed relationships between MMP-2, MMP-9 and seminal parameters.</p></div>","PeriodicalId":54391,"journal":{"name":"Journal of Mens Health","volume":"9 4","pages":"Pages 216-219"},"PeriodicalIF":0.7,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jomh.2012.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54625603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Editor's Comments 编辑的评论
IF 0.7 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2012-12-01 DOI: 10.1016/j.jomh.2012.10.001
Ajay Nehra MD (Editor-in-Chief of jmh)
{"title":"Editor's Comments","authors":"Ajay Nehra MD (Editor-in-Chief of jmh)","doi":"10.1016/j.jomh.2012.10.001","DOIUrl":"https://doi.org/10.1016/j.jomh.2012.10.001","url":null,"abstract":"","PeriodicalId":54391,"journal":{"name":"Journal of Mens Health","volume":"9 4","pages":"Page 206"},"PeriodicalIF":0.7,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jomh.2012.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136550926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Mens Health
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