Nanostructured Platform Based on Graphene-polypyrrole Composite for Immunosensor Fabrication

Andreea Cernat, Mihaela Tertiș, Claudia Nicoleta Păpară, Ede Bodoki, Robert Săndulescu
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引用次数: 9

Abstract

A hybrid graphene-polypyrrole (PPy) composite-based platform was elaborated by using nanosphere lithography structuration. This platform was further used for the antibody anti-acetaminophen immobilization in order to obtain an immunosensor for selective detection of paracetamol (acetaminophen) by using electrochemical methods. After patterning of the composite layer, the five-fold improvement of electrochemical signal was observed, suggesting that a higher amount of antibody was immobilized. This strategy permitted the ease and controlled immobilization of bioreceptors on the composite graphene-PPy platform and enhanced the sensitivity for paracetamol detection.

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基于石墨烯-聚吡咯复合材料的纳米结构平台制备免疫传感器
采用纳米球光刻技术制备了石墨烯-聚吡咯(PPy)复合材料平台。将该平台进一步用于抗对乙酰氨基酚抗体的固定,获得一种电化学方法选择性检测扑热息痛(对乙酰氨基酚)的免疫传感器。经过图案化处理后,电化学信号提高了5倍,表明固定了更多的抗体。该策略使得生物受体在复合石墨烯-聚吡啶平台上的固定更加容易和可控,并提高了对乙酰氨基酚检测的灵敏度。
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