Passive protein removal by two multipurpose lens solutions: comparison of effects on in vitro deposited and patient-worn hydrogel contact lenses

International contact lens clinic (New York, N.Y.) Pub Date : 1999-03-01 DOI:10.1016/S0892-8967(99)00020-6

Peter A Simmons PhD (FAAO), William H Ridder III PhD (OD, FAAO), Timothy B Edrington MS (OD, FAAO), Sharon Ho (OD), Kuen-Chine Lau (OD)

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引用次数: 12

Abstract

Many contact lens wearers do not perform sufficient surfactant cleaning to remove deposited protein from their lenses. In this study, the “passive” protein removal performance during soaking/disinfection of two multipurpose solutions was investigated. Both artificially deposited and patient-worn etafilcon hydrogel lenses (Surevue, Vistakon, FDA group 4) were examined. Whole lenses or lens quarters were soaked in ReNu MultiPlus (Bausch & Lomb) or Complete Comfort Plus (Allergan). After soaking for 2–24 hours, lenses were removed and solutions assayed for desorbed lysozyme by spectrophotometry. Both multipurpose solutions performed passive protein removal during soaking. Complete removed more than ReNu (means of 211.5 vs 125.3 μg/lens from patient-worn lenses, respectively, after 7 hours). Protein assay of lens pieces after soaking indicated low (<60 μg/lens) amounts of superficial protein remaining in both solution groups. The removal represents 10–50% of the total protein load on the lens and may have an impact on the overall clinical performance of these products.

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两种多用途隐形眼镜溶液的被动蛋白去除:对体外沉积和患者佩戴的水凝胶隐形眼镜的效果比较

许多隐形眼镜佩戴者没有进行足够的表面活性剂清洁来清除镜片上沉积的蛋白质。在这项研究中，研究了两种多用途溶液在浸泡/消毒过程中的“被动”蛋白质去除性能。人工沉淀和患者配戴依他非康水凝胶镜片(Surevue, Vistakon, FDA第4组)均进行了检查。将整个镜片或镜片碎片浸泡在ReNu MultiPlus (Bausch &;或Complete Comfort Plus (Allergan)。浸泡2-24小时后，取出镜片，分光光度法测定溶液解吸溶菌酶。两种多用途溶液在浸泡过程中都进行了被动的蛋白质去除。完全去除超过ReNu(7小时后患者配戴的晶状体平均分别为211.5 μg和125.3 μg/晶状体)。浸泡后的晶状体片蛋白检测显示，两组溶液中表面蛋白残留量均较低(<60 μg/晶状体)。这种去除占晶状体总蛋白质负荷的10-50%，可能对这些产品的整体临床性能产生影响。

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International contact lens clinic (New York, N.Y.)

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