Isolation and Pathogenicity Analyses on Yersinia enterocolitica from Pelteobagrus vachelli

Journal of Northeast Agricultural UniversityEnglish Edition Pub Date : 2015-06-01 DOI:10.1016/S1006-8104(15)30029-5

Zhao Jing, Wang Li

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Abstract

Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16S rDNA sequence analysis. Five virulence factors were detected, then artificial infection experiment and histopathological method were carried out. These results showed that strain YER6022 was one of Y. enterocolitica family members. In addition, ail, ystb, virF, yadA and HPIint were dectected. In artificial infection experiment, with 80% mortality and 100% morbidity, injected Pelteobagrus vachellis showed red swollen of the anus, abdomen swelling and fim bleeding. There existed serious hyperaemia and edema in kidney, spleen, intestine and liver at the light microscope. Ultrastructural observation indicated that mitochondria of the liver, kidney, spleen and intestine swelled and mitochondrial cristae broke. The data had further shed light on its pathogenicity in Pelteobagrus vachelli. It would benefit for further studies on pathogenesis of Pelteobagrus vachelli infected with Y. enterocolitica.

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巴氏海参小肠结肠炎耶尔森菌的分离及致病性分析

摘要小肠结肠炎耶尔森菌是一种重要的人畜共患病原体，可在广泛的宿主范围内引起疾病暴发。菌株YER6022从瓦切利Pelteobagrus vachelli中分离得到，通过细菌形态和16S rDNA序列分析对其进行了鉴定。检测5种毒力因子，进行人工感染实验和组织病理学检测。结果表明，YER6022是小肠结肠炎耶氏菌科成员之一。同时检测ail、ystb、virF、yadA、HPIint。在人工感染实验中，注射海参后肛门红肿，腹部肿胀，膜出血，死亡率80%，发病率100%。光镜下肾、脾、肠、肝均存在严重的充血水肿。超微结构观察显示肝、肾、脾、肠线粒体肿胀，线粒体嵴断裂。这些数据进一步阐明了其在巴氏大鼠中的致病性。为进一步研究小肠结肠炎耶氏菌感染瓦切利鼠的发病机制奠定了基础。

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Journal of Northeast Agricultural UniversityEnglish Edition

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