Simultaneous Determination of Hexoestrol, Diethylstilbestrol, Estrone and 17-Beta-estradiol in Feed by Gas Chromatography-mass Spectrometry

Journal of Northeast Agricultural UniversityEnglish Edition Pub Date : 2016-03-01 DOI:10.1016/S1006-8104(16)30031-9

Huo Feng, Li Ning, Lin Xiao-Li

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引用次数: 4

Abstract

A method was developed for the simultaneous determination of four kinds of estrogens (hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol) in feed by gas chromatography-mass spectrometry (GC-MS). After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatography-mass spectrometry. The results showed that the linear detectable ranged from 2.5 ng · mL⁻¹ to 250 ng · mL⁻¹ for hexoestrol and from 5 ng · mL⁻¹ to 500 ng · mL⁻¹ for three other estrogens with the correlation coefficients (R²) were no less than 0.990. The recoveries were in the range of 76.34%–96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation (LOQ) for all analytics were between 10 ug · kg⁻¹ and 20 ug · kg⁻¹. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.

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气相色谱-质谱法同时测定饲料中己烯雌酚、己烯雌酚、雌酮和17- β -雌二醇

建立了同时测定饲料中己雌酚、己烯雌酚、雌酮和17-雌二醇4种雌激素的气相色谱-质谱联用方法。样品经乙醚萃取，HLB相萃取柱清洗后，得到4种雌激素，并进行气相色谱-质谱联用定量分析。结果表明，己烯雌酮的线性检测范围为2.5 ~ 250 ng·mL−1，其余3种雌激素的线性检测范围为5 ~ 500 ng·mL−1，相关系数(R2)均不小于0.990。加样回收率为76.34% ~ 96.33%，相对标准偏差不大于22.7%。所有分析的定量限(LOQ)在10 ug·kg - 1和20 ug·kg - 1之间。该方法准确、灵敏，能满足饲料样品分析的实际要求。

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Journal of Northeast Agricultural UniversityEnglish Edition

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