Maciej W. Guzik, Gearóid F. Duane, Shane T. Kenny, Eoin Casey, Pawe? Mielcarek, Magdalena Wojnarowska, Kevin E. O’Connor
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引用次数: 2
Abstract
The modelling and optimization of a process for the production of the medium chain length polyhydroxyalkanoate (mcl-PHA) by the bacterium Pseudomonas putida KT2440 when fed a synthetic fatty acid mixture (SFAM) was investigated. Four novel feeding strategies were developed and tested using a constructed model and the optimum one implemented in further experiments. This strategy yielded a cell dry weight of 70.6 g l−1 in 25 h containing 38% PHA using SFAM at 5 l scale. A phosphate starvation strategy was implemented to improve PHA content, and this yielded 94.1 g l−1 in 25 h containing 56% PHA using SFAM at 5 l scale. The process was successfully operated at 20 l resulting in a cell dry weight of 91.2 g l−1 containing 65% PHA at the end of a 25-h incubation.
研究了恶臭假单胞菌KT2440在合成脂肪酸混合物(sfm)条件下生产中链长聚羟基烷酸酯(mcl-PHA)的工艺模型和优化。采用构建的模型对四种新的喂养策略进行了测试，并在进一步的实验中实施了最优喂养策略。该策略在25小时内产生了70.6 g l−1的细胞干重，其中含有38%的PHA。采用磷酸盐饥饿策略来提高PHA含量，在5 l的sfm中，25 h的产量为94.1 g l−1,PHA含量为56%。该工艺在20 l条件下成功操作，在25 h孵育结束时，细胞干重为91.2 g l−1，含65% PHA。
期刊介绍：
Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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