{"title":"Web alert: Two-phase biocatalysis","authors":"Lawrence P. Wackett","doi":"10.1111/1751-7915.14314","DOIUrl":null,"url":null,"abstract":"<p>Microfluidic droplets</p><p>\n https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616014/\n </p><p>This review article covers two-phase biocatalysis in microfluidic droplets. This often serves to overcome mass transport issues that can limit enzyme catalysis with water-insoluble compounds.</p><p>Two-step organic synthesis biocatalysis</p><p>\n https://pubs.acs.org/doi/10.1021/acs.oprd.6b00232\n </p><p>This study used whole-cell catalysts in a microaqueous phase within organic solvents to catalyse multiple enzymatic steps for making a pharmaceutical intermediate.</p><p>Flow biocatalysis</p><p>\n https://www.frontiersin.org/articles/10.3389/fctls.2023.1154452/full\n </p><p>This review covered new developments in flow biocatalysis during the years of 2020–2022.</p><p>Encapsulated <i>Pseudomonas</i> biotransformation</p><p>\n https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02073-7\n </p><p>In this report, the authors describe optimization of (S)-2-hydroxypropiophenone synthesis by free and encapsulated cells of <i>P. putida</i>.</p><p>Biocatalysis methods</p><p>\n https://www.nature.com/articles/s43586-021-00044-z\n </p><p>This is a broad and extensive review of biocatalysis that includes many aspects for using enzymes to synthesis commercial chemicals.</p><p>Pickering emulsions with yeast enzyme</p><p>\n https://pubs.acs.org/doi/10.1021/acssuschemeng.6b01776\n </p><p>This article describes the use of a small amount of solid particle emulsifier to make a Pickering emulsion system to catalyse reactions with <i>Candida antarctica</i> lipase B.</p><p>Pharma biocatalysis</p><p>\n https://www.almacgroup.com/knowledge/wp-content/uploads/sites/10/2021/01/API_Practical-Methods-for-Biocatalysis-and-Biotransformations-2_Article-2.pdf\n </p><p>This review chapter focuses on company-based biocatalysis projects.</p><p>Microbial biocatalysis</p><p>\n https://www.mdpi.com/journal/catalysts/special_issues/microbes_biocatal\n </p><p>This links to a special issue of the journal “Catalysts” that includes examples of two-phase microbial and enzyme biocatalysis.</p><p>Diene epoxidation by a <i>Pseudomonas</i></p><p>\n https://www.sciencedirect.com/science/article/abs/pii/0141022986900761\n </p><p>This article highlights the production of 7,8-epoxy-1-octene by non-growing <i>Pseudomonas putida</i> PpG6 in two-phase catalytic reaction.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"16 8","pages":"1702"},"PeriodicalIF":4.8000,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.14314","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1751-7915.14314","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
This review article covers two-phase biocatalysis in microfluidic droplets. This often serves to overcome mass transport issues that can limit enzyme catalysis with water-insoluble compounds.
Two-step organic synthesis biocatalysis
https://pubs.acs.org/doi/10.1021/acs.oprd.6b00232
This study used whole-cell catalysts in a microaqueous phase within organic solvents to catalyse multiple enzymatic steps for making a pharmaceutical intermediate.
This article describes the use of a small amount of solid particle emulsifier to make a Pickering emulsion system to catalyse reactions with Candida antarctica lipase B.
期刊介绍:
Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes