{"title":"The role of senescence-associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar","authors":"Hui He, Xue-Qin Song, Cheng Jiang, Ying-Li Liu, Dian Wang, Shuang-Shuang Wen, Guo-Hua Chai, Shu-Tang Zhao, Meng-Zhu Lu","doi":"10.1111/jipb.13195","DOIUrl":null,"url":null,"abstract":"<p>Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated <i>PagSAG101a</i>, the homolog of <i>Arabidopsis thaliana SAG101</i>, from a hybrid poplar (<i>Populus alba</i> × <i>Populus glandulosa</i>) clone 84K and investigated its role in secondary xylem development. <i>PagSAG101a</i> was expressed predominantly in lignified stems and localized in the nucleus. Compared with non-transgenic 84K plants, transgenic plants overexpressing <i>PagSAG101a</i> displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for <i>PagSAG101a</i> knock-out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of <i>PagSAG101a</i> and mediate the regulation of xylem differentiation. Our results support that PagSAG101a, downstream of PagC3H17, functions in wood development.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3000,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13195","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13195","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar (Populus alba × Populus glandulosa) clone 84K and investigated its role in secondary xylem development. PagSAG101a was expressed predominantly in lignified stems and localized in the nucleus. Compared with non-transgenic 84K plants, transgenic plants overexpressing PagSAG101a displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for PagSAG101a knock-out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of PagSAG101a and mediate the regulation of xylem differentiation. Our results support that PagSAG101a, downstream of PagC3H17, functions in wood development.
木本植物的木材是由次生木质部通过形成层细胞的增殖和分化积累而成的。确定参与这一过程的监管机构仍然是一项具有挑战性的任务。本研究从白杨(Populus alba × Populus glandullosa)杂交无性系84K中分离出拟南芥SAG101的同源基因PagSAG101a,并对其在次生木质部发育中的作用进行了研究。PagSAG101a主要在木质化茎中表达,并定位于细胞核中。与非转基因84K植株相比,过表达PagSAG101a的转基因植株的株高、节间数、茎粗、木质部宽度和次生细胞壁厚度均有所增加,而敲除PagSAG101a的植株则表现出相反的表型。转录组分析显示,在木质部形成过程中,控制形成层细胞分裂活性和随后的次生细胞壁沉积的差异表达基因富集。此外,串联CCCH锌指蛋白PagC3H17可以与PagSAG101a启动子结合,介导木质部分化的调节,而PagC3H17正调控杨树次生木质部宽度和次生壁增厚。我们的研究结果支持PagC3H17下游的PagSAG101a在木材发育中起作用。
期刊介绍:
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.