Tool box: Plasmids for the expression or knockdown of human ARF Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria with N-myristoyltransferases

Abstract

This article is intended to inform researchers about a collection of » 200 plasmids recently made available through Addgene (www.addgene.com), that were generated to facilitate the study of human ARF family GTPases, including all 5 ARF and 2 SAR and an incomplete collection of ARF-like (ARL) proteins. They fall into 3 groups based upon usage; (1) ARF family GTPase expression in mammalian or bacterial cells, (2) N-myristoyltransferase co-expression in bacteria