Vacuolar MATE/DTX protein-mediated cucurbitacin C transport is co-regulated with bitterness biosynthesis in cucumber

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2023-02-02 DOI:10.1111/nph.18786

Yongshuo Ma, Dawei Li, Yang Zhong, Xiaohan Wang, Legong Li, Anne Osbourn, William J. Lucas, Sanwen Huang, Yi Shang

{"title":"Vacuolar MATE/DTX protein-mediated cucurbitacin C transport is co-regulated with bitterness biosynthesis in cucumber","authors":"Yongshuo Ma, Dawei Li, Yang Zhong, Xiaohan Wang, Legong Li, Anne Osbourn, William J. Lucas, Sanwen Huang, Yi Shang","doi":"10.1111/nph.18786","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n </p><ul>\n \n <li>Membrane-localized transporters constitute important components for specialized metabolism in plants. However, due to the vast array of specialized metabolites produced by plants, and the large families of transporter genes, knowledge about the intracellular and intercellular transport of plant metabolites is still in its infancy.</li>\n \n <li>Cucurbitacins are bitter and defensive triterpenoids produced mainly in the cucurbits. Using a comparative genomics and multi-omics approach, a MATE gene (<i>CsMATE1</i>), physically clustered with cucurbitacin C (CuC) biosynthetic genes, was identified and functionally shown to sequester CuC in cucumber leaf mesophyll cells.</li>\n \n <li>Notably, the CuC transport process is strictly co-regulated with CuC biosynthesis. CsMATE1 clustering with bitterness biosynthesis genes may provide benefits and a basis for this feedback regulation on CuC sequestration and biosynthesis.</li>\n \n <li>Identification of transport systems for plant-specialized metabolites can accelerate the metabolic engineering of high-value-added compounds by simplifying their purification process.</li>\n </ul>\n </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":8.3000,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/nph.18786","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 2

Abstract

Membrane-localized transporters constitute important components for specialized metabolism in plants. However, due to the vast array of specialized metabolites produced by plants, and the large families of transporter genes, knowledge about the intracellular and intercellular transport of plant metabolites is still in its infancy.
Cucurbitacins are bitter and defensive triterpenoids produced mainly in the cucurbits. Using a comparative genomics and multi-omics approach, a MATE gene (CsMATE1), physically clustered with cucurbitacin C (CuC) biosynthetic genes, was identified and functionally shown to sequester CuC in cucumber leaf mesophyll cells.
Notably, the CuC transport process is strictly co-regulated with CuC biosynthesis. CsMATE1 clustering with bitterness biosynthesis genes may provide benefits and a basis for this feedback regulation on CuC sequestration and biosynthesis.
Identification of transport systems for plant-specialized metabolites can accelerate the metabolic engineering of high-value-added compounds by simplifying their purification process.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

液泡MATE/DTX蛋白介导的葫芦素C转运与黄瓜苦味生物合成共同调控

膜定位转运体是植物特化代谢的重要组成部分。然而，由于植物产生的代谢物种类繁多，转运基因家族众多，关于植物代谢物的细胞内和细胞间运输的知识仍处于起步阶段。葫芦素是一种苦味的防御性三萜，主要产生于葫芦中。利用比较基因组学和多组学方法，鉴定了一个与葫芦素C (CuC)生物合成基因物理聚类的MATE基因(CsMATE1)，并在功能上证明了该基因在黄瓜叶肉细胞中对CuC的隔离作用。值得注意的是，CuC的转运过程与CuC的生物合成密切相关。具有苦味生物合成基因的CsMATE1聚类可能为这种对CuC固存和生物合成的反馈调控提供益处和依据。鉴定植物特化代谢物的运输系统可以通过简化其纯化过程来加速高附加值化合物的代谢工程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.