In vitro culture of cryopreserved caprine ovarian tissue pieces and isolated follicles

Abstract

The objective of the present study was to evaluate the effects of cryopreservation of goat preantral follicles, in situ and isolated, using dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Follicles were cryopreserved and cultured in vitro, and follicle viability was assessed by Trypan Blue staining before and after culture. The viability of follicles cryopreserved in situ using 1.5 M DMSO (64% ± 5.3 and 60% ± 7.4) or 3.0 M EG (58% ± 3.7 and 45% ± 4.4) after days 1 and 5, respectively, of culture was lower (p 0.05). For isolated follicles, the percentage of viable fresh follicles was higher than follicles cryopreserved in EG on days 1 and 5 of culture, and in DMSO on day 1 of culture. Follicle diameter was not altered during culture, both for fresh and cryopreserved follicles and when follicles were cultured in situ or isolated. In conclusion, caprine preantral follicles were successfully cryopreserved, especially in situ, and follicle viability after isolation was similar between fresh and cryopreserved follicles after 1 day of culture, when DMSO was used. © Mary Ann Liebert, Inc.