ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF.

IF 2.4 3区物理与天体物理 Q2 INSTRUMENTS & INSTRUMENTATION Journal of Synchrotron Radiation Pub Date : 2022-03-01 Epub Date: 2022-02-22 DOI:10.1107/S1600577522000984

Max Nanao, Shibom Basu, Ulrich Zander, Thierry Giraud, John Surr, Matias Guijarro, Mario Lentini, Franck Felisaz, Jeremy Sinoir, Christian Morawe, Amparo Vivo, Antonia Beteva, Marcus Oscarsson, Hugo Caserotto, Fabien Dobias, David Flot, Didier Nurizzo, Jonathan Gigmes, Nicolas Foos, Ralf Siebrecht, Thomas Roth, Pascal Theveneau, Olof Svensson, Gergely Papp, Bernard Lavault, Florent Cipriani, Ray Barrett, Carole Clavel, Gordon Leonard

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Abstract

ID23-2 is a fixed-energy (14.2 keV) microfocus beamline at the European Synchrotron Radiation Facility (ESRF) dedicated to macromolecular crystallography. The optics and sample environment have recently been redesigned and rebuilt to take full advantage of the upgrade of the ESRF to the fourth generation Extremely Brilliant Source (ESRF-EBS). The upgraded beamline now makes use of two sets of compound refractive lenses and multilayer mirrors to obtain a highly intense (>10¹³ photons s^-1) focused microbeam (minimum size 1.5 µm × 3 µm full width at half-maximum). The sample environment now includes a FLEX-HCD sample changer/storage system, as well as a state-of-the-art MD3Up high-precision multi-axis diffractometer. Automatic data reduction and analysis are also provided for more advanced protocols such as synchrotron serial crystallographic experiments.

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ID23-2：ESRF 用于大分子晶体学的自动化高性能微聚焦光束线。

ID23-2是欧洲同步辐射设施（ESRF）的一条固定能量（14.2 keV）微聚焦光束线，专门用于大分子晶体学研究。为了充分利用欧洲同步辐射设施升级为第四代极亮光源（ESRF-EBS）的优势，最近对光学系统和样品环境进行了重新设计和改造。升级后的光束线现在使用两组复合折射透镜和多层反射镜来获得高强度（>1013 光子 s-1）聚焦微光束（最小尺寸为 1.5 微米 × 3 微米半最大全宽）。样品环境现在包括一个 FLEX-HCD 样品更换器/存储系统，以及一个最先进的 MD3Up 高精度多轴衍射仪。还为同步辐射串行晶体学实验等更先进的方案提供了自动数据还原和分析功能。

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来源期刊

Journal of Synchrotron Radiation 物理-光学

CiteScore

5.10

自引率

12.00%

发文量

289

审稿时长

4-8 weeks

期刊介绍： Synchrotron radiation research is rapidly expanding with many new sources of radiation being created globally. Synchrotron radiation plays a leading role in pure science and in emerging technologies. The Journal of Synchrotron Radiation provides comprehensive coverage of the entire field of synchrotron radiation and free-electron laser research including instrumentation, theory, computing and scientific applications in areas such as biology, nanoscience and materials science. Rapid publication ensures an up-to-date information resource for scientists and engineers in the field.