Usefulness of anti-phosphohistone H3 immunoreactivity to determine mitotic rate in gastrointestinal stromal tumors

Basic and applied pathology Pub Date : 2012-12-19 DOI:10.1111/baap.12003

Ahrong Kim, Dong Han Im, Kyungbin Kim, Jee Yeon Kim, Mee Young Sol, Jeong Hee Lee, Kyung Un Choi

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引用次数: 2

Abstract

Background and aim

The biologic behavior of gastrointestinal stromal tumors (GIST) is a topic of continuing controversy. The assessment of accurate mitotic figures is known to be one of the major indicators for patients with GIST. However, it is not always easy to search for mitotic figures (MFs) and count them accurately on hematoxylin and eosin stained (H&E) slides.

Methods

In this study, phopho-histone H3 (PHH3), which is a recently described specific mitosis marker, was immunohistochemically examined and compared to the mitotic count on H&E sections (H&E-MI) and Ki-67 expression (Ki-67 PI). A hundred cases of histologically confirmed GISTs were reviewed based on counting MF on H&E slides. PHH3 positive MFs (PHH3-MI) were counted in the same way, and the Ki-67 PI was calculated for each case.

Results

A strong correlation was found between PHH3-MI and H&E-MI. Recurrence-free survival was correlated with risk category by National Institutes of Health (NIH) consensus criteria (P = 0.017), mucosal invasion (P = 0.005), H&E-MI (P = 0.002), Ki-67 PI (P = 0.005), and PHH3-MI (P = 0.000). None of these factors was an independent prognostic factor.

Conclusions

Among GISTs, PHH3 staining was primarily found to support grading by facilitating mitotic counting, and it might have a prognostic value in GISTs.

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抗磷酸组蛋白H3免疫反应性测定胃肠道间质瘤有丝分裂率的有效性

背景与目的胃肠道间质瘤(GIST)的生物学行为一直是一个有争议的话题。评估准确的有丝分裂数字是已知的GIST患者的主要指标之一。然而，在苏木精和伊红染色(H&E)载玻片上搜索有丝分裂象(MFs)并准确计数并不总是容易的。方法采用免疫组织化学方法对新近发现的特异性有丝分裂标志物磷酸组蛋白H3 (PHH3)进行检测，并与H&E切片上的有丝分裂计数(H&E- mi)和Ki-67的表达(Ki-67 PI)进行比较。根据H&E载玻片上的MF计数，回顾了100例组织学证实的gist病例。同样方法计算PHH3阳性mf (PHH3- mi)，并计算Ki-67 PI。结果PHH3-MI与H&E-MI有较强的相关性。无复发生存率与美国国立卫生研究院(NIH)共识标准的风险分类(P = 0.017)、粘膜侵袭(P = 0.005)、H&E-MI (P = 0.002)、Ki-67 PI (P = 0.005)和PHH3-MI (P = 0.000)相关。这些因素都不是独立的预后因素。结论在gist中，PHH3染色主要通过促进有丝分裂计数来支持分级，并且可能在gist中具有预后价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Basic and applied pathology

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