HYDROGEN-DEUTERIUM EXCHANGE AND ULTRAVIOLET RESONANCE RAMAN SPECTROSCOPY OF BACTERIA IN A COMPLEX FOOD MATRIX†

GREGORY P. HARHAY, GREGORY R. SIRAGUSA
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引用次数: 5

Abstract

Abstract The rapid identification and quantification of pathogenic foodborne bacteria is a national research priority. Data collected with a new spectrochemical method suggests that resonance Raman spectroscopy can rapidly and selectively identify and quantify bacteria in foods and other complex biomatrices. This method utilizes hydrogen-deuterium exchange (HDE) to resolve the spectral fingerprints of individual components in the overlapped regions of the ultraviolet resonance Raman spectra of heterogeneous samples. We illustrate this concept with HDE-induced changes in the spectra of bacteria and beef carcass wash samples. The data presented here suggest that the combination of ultraviolet resonance Raman spectroscopy and HDE can potentially establish the identities and quantities of bacteria in heterogeneous samples within seconds to minutes.

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复杂食物基质中细菌的氢-氘交换和紫外共振拉曼光谱研究
致病性食源性细菌的快速鉴定和定量是国家研究的重点。用一种新的光谱化学方法收集的数据表明,共振拉曼光谱可以快速、选择性地识别和定量食品和其他复杂生物基质中的细菌。该方法利用氢-氘交换(HDE)来解析非均质样品紫外共振拉曼光谱重叠区域中单个组分的光谱指纹图谱。我们用hde诱导的细菌和牛肉胴体洗涤样品光谱的变化来说明这一概念。本文提供的数据表明,紫外共振拉曼光谱和HDE的结合可以在几秒到几分钟内确定异质样品中细菌的身份和数量。
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Journal of Rapid Methods and Automation in Microbiology
Journal of Rapid Methods and Automation in Microbiology 生物-生物工程与应用微生物
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